NDLI: Neurofilament protein is phosphorylated in the squid giant axon.

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Neurofilament protein is phosphorylated in the squid giant axon.

Content Provider	Scilit
Author	Pant, H. C. Shecket, G. Gainer, H. Lasek, R. J.
Copyright Year	1978
Description	We have observed the phosphorylation of neurofilament protein from squid axoplasm. Phosphorylation is demonstrated by 32P labeling of protein during incubation of axoplasm with [gamma-32P]ATP. When the labeled proteins are separated by SDS-polyacrylamide gel electrophoresis (SDS-PAGE), two bands, at 2.0 x 10(5) daltons and greater than 4 x 10(5) daltons, contain the bulk of the 32P. The 2.0 x 10(5)-dalton phosphorylated polypeptide comigrates on SDS-PAGE with one of the subunits of squid neurofilament protein. Both major phosphorylated polypeptides co-fractionate with neurofilaments in discontinuous sucrose gradient centrifugation and on gel filtration chromatography on Sepharose 4B. The protein-phosphate bond behaves like a phospho-ester, and labeled phospho-serine is identified in an acid hydrolysate of the protein. The generality of this phenomenon in various species and its possible physiological significance are discussed.
Related Links	http://europepmc.org/articles/pmc2110134?pdf=render https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2110134/pdf
Ending Page	R27
Page Count	5
Starting Page	R23
DOI	10.1083/jcb.78.2.r23
Journal	Journal of Cell Biology
Issue Number	2
Volume Number	78
Language	English
Publisher	Rockefeller University Press
Publisher Date	1978-08-01
Access Restriction	Open
Subject Keyword	Biology Protein Page Squid Neurofilament Polypeptide Axoplasm Phosphorylated Labeled Daltons Journal: Journal of Cell Biology (Vol- 78, Issue- 2)
Content Type	Text

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