NDLI: Autonomous microfluidic multi-channel chip for real-time PCR with integrated liquid handling

Content Provider	Springer Nature Link
Author	Frey, Olivier Bonneick, Sonja Hierlemann, Andreas Lichtenberg, Jan
Copyright Year	2007
Abstract	We report on a novel, polymer-based, multi-channel device for polymerase chain reaction that combines, for the first time, rapid sample processing in less than 5 min with high throughput at low costs. This is achieved by sample shuttling, during which submicroliter sample plugs (∼100 nl) are oscillated rapidly over three constant-temperature zones by pneumatic actuation with integrated system. The accuracy and the speed of the liquid handling have been significantly increased, while the design of the device can be kept very simple and allows for mass production using conventional low-cost polymer fabrication processes. Massive parallelization can lead to a throughput up to 100 samples in 10 min including the preparation time. The amplification can be optically monitored by means of online fluorescence detection. Successful real-time PCR and the determination of the threshold cycle, C t, using the developed device were demonstrated with plasmid DNA in a fluorescent real-time format.
Starting Page	711
Ending Page	718
Page Count	8
File Format	PDF
ISSN	13872176
Journal	Biomedical Microdevices
Volume Number	9
Issue Number	5
e-ISSN	15728781
Language	English
Publisher	Springer US
Publisher Date	2007-05-16
Publisher Place	Boston
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	PCR Microchip PCR Sample shuttling PDMS Diffuser nozzle structures Engineering Fluid Dynamics Nanotechnology Biophysics/Biomedical Physics Biomedical Engineering
Content Type	Text
Resource Type	Article
Subject	Nanoscience and Nanotechnology Molecular Biology Biomedical Engineering

Sl.	Authority	Responsibilities	Communication Details
1	Ministry of Education (GoI), Department of Higher Education	Sanctioning Authority	https://www.education.gov.in/ict-initiatives
2	Indian Institute of Technology Kharagpur	Host Institute of the Project: The host institute of the project is responsible for providing infrastructure support and hosting the project	https://www.iitkgp.ac.in
3	National Digital Library of India Office, Indian Institute of Technology Kharagpur	The administrative and infrastructural headquarters of the project	Dr. B. Sutradhar bsutra@ndl.gov.in
4	Project PI / Joint PI	Principal Investigator and Joint Principal Investigators of the project	Dr. B. Sutradhar bsutra@ndl.gov.in Prof. Saswat Chakrabarti will be added soon
5	Website/Portal (Helpdesk)	Queries regarding NDLI and its services	support@ndl.gov.in
6	Contents and Copyright Issues	Queries related to content curation and copyright issues	content@ndl.gov.in
7	National Digital Library of India Club (NDLI Club)	Queries related to NDLI Club formation, support, user awareness program, seminar/symposium, collaboration, social media, promotion, and outreach	clubsupport@ndl.gov.in
8	Digital Preservation Centre (DPC)	Assistance with digitizing and archiving copyright-free printed books	dpc@ndl.gov.in
9	IDR Setup or Support	Queries related to establishment and support of Institutional Digital Repository (IDR) and IDR workshops	idr@ndl.gov.in

Real Time PCR on Disposable PDMS Chip with a Miniaturized Thermal Cycler

Real-time PCR array chip with capillary-driven sample loading and reactor sealing for point-of-care applications

Cross-Talk Problem on a Fluorescence Multi-Channel Microfluidic Chip System

Miniature real time PCR on chip with multi-channel fiber optical fluorescence detection module

A disposable lab-on-a-chip platform with embedded fluid actuators for active nanoliter liquid handling

Microfabrication of cylindrical microfluidic channel networks for microvascular research

A portable pressure pump for microfluidic lab-on-a-chip systems using a porous polydimethylsiloxane (PDMS) sponge

An integrated microfluidic PCR system with immunomagnetic nanoparticles for the detection of bacterial pathogens

Plug-and-play, infrared, laser-mediated PCR in a microfluidic chip

Autonomous microfluidic multi-channel chip for real-time PCR with integrated liquid handling

Similar Documents

Real Time PCR on Disposable PDMS Chip with a Miniaturized Thermal Cycler

Real-time PCR array chip with capillary-driven sample loading and reactor sealing for point-of-care applications

Cross-Talk Problem on a Fluorescence Multi-Channel Microfluidic Chip System

Miniature real time PCR on chip with multi-channel fiber optical fluorescence detection module

A disposable lab-on-a-chip platform with embedded fluid actuators for active nanoliter liquid handling

Microfabrication of cylindrical microfluidic channel networks for microvascular research

A portable pressure pump for microfluidic lab-on-a-chip systems using a porous polydimethylsiloxane (PDMS) sponge

An integrated microfluidic PCR system with immunomagnetic nanoparticles for the detection of bacterial pathogens

Plug-and-play, infrared, laser-mediated PCR in a microfluidic chip

Autonomous microfluidic multi-channel chip for real-time PCR with integrated liquid handling