NDLI: Microfluidic self-assembly of live Drosophila embryos for versatile high-throughput analysis of embryonic morphogenesis

Content Provider	Springer Nature Link
Author	Dagani, Gabriel T. Monzo, Kate Fakhoury, Jean R. Chen, Chung Chu Sisson, John C. Zhang, Xiaojing
Copyright Year	2007
Abstract	A method for assembling Drosophila embryos in a microfluidic device was developed for studies of thermal perturbation of early embryonic development. Environmental perturbation is a complimentary method to injection of membrane-impermeable macromolecules for assaying genetic function and investigating robustness in complex biochemical networks. The development of a high throughput method for perturbing embryos would facilitate the isolation and mapping of signaling pathways. We immobilize Drosophila embryos inside a microfluidic device on minimal potential-energy wells created through surface modification, and thermally perturb these embryos using binary laminar flows of warm and cold solutions. We self-assemble embryos onto oil adhesive pads with an alcohol surfactant carrier fluid (detachment: 0.1 mL/min), and when the surfactant is removed, the embryo-oil adhesion increases to ∼25 mL/min flow rates, which allows for high velocities required for sharp gradients of thermal binary flows. The microfluidic thermal profile was numerically characterized by simulation and experimentally characterized by fluorescence thermometry. The effects of thermal perturbation were observed to induce abnormal morphogenetic movements in live embryos by using time-lapse differential interference contrast (DIC) microscopy.
Starting Page	681
Ending Page	694
Page Count	14
File Format	PDF
ISSN	13872176
Journal	Biomedical Microdevices
Volume Number	9
Issue Number	5
e-ISSN	15728781
Language	English
Publisher	Springer US
Publisher Date	2007-05-17
Publisher Place	Boston
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Microfluidic Self-assembly (Polydimethylsiloxane) PDMS Drosophila embryo Thermal perturbation Embryogenesis Cellularization Engineering Fluid Dynamics Nanotechnology Biophysics/Biomedical Physics Biomedical Engineering
Content Type	Text
Resource Type	Article
Subject	Nanoscience and Nanotechnology Molecular Biology Biomedical Engineering

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