NDLI: Assembly of skeletal muscle cells on a Si-MEMS device and their generative force measurement

Content Provider	Springer Nature Link
Author	Shimizu, Kazuri Sasaki, Hikaru Hida, Hirotaka Fujita, Hideaki Obinata, Kei Shikida, Mitsuhiro Nagamori, Eiji
Copyright Year	2009
Abstract	We have fabricated a simple Si-MEMS device consisting of a microcantilever and a base to measure active tension generated by skeletal muscle myotubes derived from murine myoblast cell line C2C12. We have developed a fabrication process for integration of myotubes onto the device. To position myotubes over the gap between the cantilever and the base without damage due to mechanical peeling or the use of an enzymatic reaction, we cultured myotubes on poly-N-isopropylacrylamide (PNIPAAm) as a sacrifice layer. By means of immune staining of α-actinin, it was confirmed that a myotube micropatterned onto the device bridged the gap between the cantilever and the base. After 7d differentiation, the myotube was actuated by electrical stimulation. The active tension generated by the myotube was evaluated by measuring the bending of the cantilever using image processing. On twitch stimulation, the myotube on the device contracted and generated active tension in response to the electrical signals. On tetanus tension measurement, approximately 1.0 μN per single myotube was obtained. The device developed here can be used in wide area of in vitro skeletal muscle studies, such as drug screening, physiology, regenerative medicine, etc.
Starting Page	247
Ending Page	252
Page Count	6
File Format	PDF
ISSN	13872176
Journal	Biomedical Microdevices
Volume Number	12
Issue Number	2
e-ISSN	15728781
Language	English
Publisher	Springer US
Publisher Date	2009-11-27
Publisher Place	Boston
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Bio-MEMS Skeletal muscle C2C12 Active tension Poly-N-isopropylacrylamide Biomedical Engineering Biophysics and Biological Physics Nanotechnology Engineering Fluid Dynamics
Content Type	Text
Resource Type	Article
Subject	Nanoscience and Nanotechnology Molecular Biology Biomedical Engineering

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