NDLI: Active and biomimetic nanofilters for selective protein separation

Content Provider	Springer Nature Link
Author	Goyal, Swati Kim, Young tae Li, Yan Iqbal, Samir M.
Copyright Year	2009
Abstract	Selective protein channels in cell and nuclear membranes act as gateways to control the passage of molecules across. The selectivity of these channels stems from attractive potentials of the binding sites in the transmembrane proteins. These channels can filter out small volume of solutions with high precision. Motivated from this phenomenon, we report biomimetic facilitated transport modality to selectively separate a target molecule from a mixture of molecules. The attractive potential is generated by specific antibodies immobilized inside 15 nm diameter polycarbonate nanochannels. Two proteins with similar physicochemical properties (Bovine Serum Albumin 66 kDa, and Human Hemoglobin 65 kDa) are chosen as model molecules. The protein molecules are mixed in ratios of 1:1, 1:20 and 1:40 (Hb:BSA), and separation of molecules is demonstrated. The selectivity of membrane can be switched from Hb to BSA by changing the immobilized antibody inside the membrane channels. This approach can be used to selectively enrich any target molecule from a complex sample to enhance signal-to-noise ratio for early disease diagnosis.
Starting Page	317
Ending Page	324
Page Count	8
File Format	PDF
ISSN	13872176
Journal	Biomedical Microdevices
Volume Number	12
Issue Number	2
e-ISSN	15728781
Language	English
Publisher	Springer US
Publisher Date	2010-01-08
Publisher Place	Boston
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Bio-separation technology Nanofiltration Nanochannels BSA Hb Engineering Fluid Dynamics Nanotechnology Biophysics and Biological Physics Biomedical Engineering
Content Type	Text
Resource Type	Article
Subject	Nanoscience and Nanotechnology Molecular Biology Biomedical Engineering

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