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| Content Provider | Springer Nature Link |
|---|---|
| Author | Jeon, Hojeong Lee, Yongku Jin, Songwan Koo, Sangmo Lee, Chang Soo Yoo, Jung Yul |
| Copyright Year | 2009 |
| Abstract | A microfluidic device to quantify bacterial chemotaxis has been proposed, which generates a linear concentration gradient of chemoattractant in the main channel only by convective and molecular diffusion, and which enables the bacteria to enter the main channel in a single file by hydrodynamic focusing technique. The trajectory of each bacterium in response to the concentration gradient of chemoattractant is photographed by a CCD camera and its velocity is acquired by a simple PTV (Particle Tracking Velocimetry) algorithm. An advantage of this assay is to measure the velocity of a single bacterium and to quantify the degree of chemotaxis by analyzing the frequency of velocities concurrently. Thus, the parameter characterizing the motility of wild-type Escherichia coli strain RP437 in response to various concentration gradients of L-aspartate is obtained in such a manner that the degree of bacterial chemotaxis is quantified on the basis of a newly proposed Migration Index. |
| Starting Page | 1135 |
| Ending Page | 1143 |
| Page Count | 9 |
| File Format | |
| ISSN | 13872176 |
| Journal | Biomedical Microdevices |
| Volume Number | 11 |
| Issue Number | 5 |
| e-ISSN | 15728781 |
| Language | English |
| Publisher | Springer US |
| Publisher Date | 2009-06-23 |
| Publisher Place | Boston |
| Access Restriction | One Nation One Subscription (ONOS) |
| Subject Keyword | Chemotaxis Bacteria Microchannel Linear concentration gradient Hydrodynamic focusing Engineering Fluid Dynamics Nanotechnology Biophysics and Biological Physics Biomedical Engineering |
| Content Type | Text |
| Resource Type | Article |
| Subject | Nanoscience and Nanotechnology Molecular Biology Biomedical Engineering |
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