NDLI: Dielectrophoresis assisted concentration of micro-particles and their rapid quantitation based on optical means

Content Provider	Springer Nature Link
Author	Ghubade, Anil Mandal, Swarnasri Chaudhury, Rahul Singh, Rajeev Kumar Bhattacharya, Shantanu
Copyright Year	2009
Abstract	The detection and counting of micro particles having sizes comparable to biological entities can provide a tremendous impetus to rapid diagnostics and clinical applications. MEMS technology has already been used in capture and detection of such micron size entities in miniscule concentrations. For this purpose a concentration step is normally added prior to the detection process. A variety of methodologies are used for quantization of such micron size particles/entities including change in permittivity, medium impedance, magnetic permeability and other means. Although optical studies have been extensively performed prior to this, it has not been used for quantization of the micro particles. We have designed, developed and characterized a MEMS counter which captures micron size fluorescent beads using delectrophoresis (DEP) and monitors their accumulation in a 12 μm × 230 μm size channel and monitors this accumulation as growth of overall fluorescence. The field is generated by a set of finely placed interdigitated microelectrodes. As we apply an alternating voltage at 10 Vpp for a range of different frequencies we are able to capture the flowing beads and concentrate them by several orders of magnitude. This is also followed by their quantification in terms of growing fluorescence signal. For quantitating the fluorescence values a CCD (charge couple device) module fitted over an inverted fluorescence microscope is used that visualizes the whole capture process and a Labview based image acquisition software simultaneously calculates the signal intensity over these frames and arranges it temporally. Our work will have tremendous utility in developing a rapid bacterial counting procedure and will be a valuable tool in microbiological laboratories.
Starting Page	987
Ending Page	995
Page Count	9
File Format	PDF
ISSN	13872176
Journal	Biomedical Microdevices
Volume Number	11
Issue Number	5
e-ISSN	15728781
Language	English
Publisher	Springer US
Publisher Date	2009-05-05
Publisher Place	Boston
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Dielectrophoresis Quantification Fluorescence Microbeads Microbiological tool Engineering Fluid Dynamics Nanotechnology Biophysics and Biological Physics Biomedical Engineering
Content Type	Text
Resource Type	Article
Subject	Nanoscience and Nanotechnology Molecular Biology Biomedical Engineering

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1	Ministry of Education (GoI), Department of Higher Education	Sanctioning Authority	https://www.education.gov.in/ict-initiatives
2	Indian Institute of Technology Kharagpur	Host Institute of the Project: The host institute of the project is responsible for providing infrastructure support and hosting the project	https://www.iitkgp.ac.in
3	National Digital Library of India Office, Indian Institute of Technology Kharagpur	The administrative and infrastructural headquarters of the project	Dr. B. Sutradhar bsutra@ndl.gov.in
4	Project PI / Joint PI	Principal Investigator and Joint Principal Investigators of the project	Dr. B. Sutradhar bsutra@ndl.gov.in Prof. Saswat Chakrabarti will be added soon
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