NDLI: Magneto-Hydrodynamic Fractionation (MHF) for continuous and sheathless sorting of high-concentration paramagnetic microparticles

Content Provider	Springer Nature Link
Author	Kumar, Vikash Rezai, Pouya
Copyright Year	2017
Abstract	Sorting cells, microorganisms and particles from a solution is of paramount importance in many biological applications. An ideal sorting device should work at high throughput, involve simple design, avoid energy consumption, operate without a diluting sheath flow and perform separation with high purity. However, currently available sorting methods such as pinched flow fractionation, hydrodynamic filtration, magnetophoresis and deterministic lateral displacement meet only a few of the above-mentioned characteristics. In this paper, we report a hybrid technique combining magnetic focusing of particles in a thin microchannel and their hydrodynamic fractionation at a downstream expansion region, to devise a sheathless and high-throughput Magneto-Hydrodynamic Fractionation (MHF) method. First, sheathless magnetic focusing of 11 μm microparticles against the wall of the thin microchannel was investigated over a wide range of flow rates (0.5–5 mL h−1). Then, a mixture of 5 μm and 11 μm paramagnetic particles was injected into the device at a flow rate of 5 mL h−1 to demonstrate their sorting. Both of these magnetic particles were aligned along the wall of the channel and hence focused in the device, however their centers were lying on different streamlines due to their different sizes. Therefore, they were separated into distinct streamlines upon entering into the expansion region. Using this device, we achieved a high throughput sorting of more than 104 particles per second with an approximate on-chip fractionation purity of 98%. This technique has a great potential for separation of more than two magnetic particles for application in immunomagnetic affinity-based sorting of multiple biological substances.
Starting Page	1
Ending Page	9
Page Count	9
File Format	PDF
ISSN	13872176
Journal	Biomedical Microdevices
Volume Number	19
Issue Number	2
e-ISSN	15728781
Language	English
Publisher	Springer US
Publisher Date	2017-05-02
Publisher Place	New York
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Microfluidic sorting Microparticles sorting Magnetophoresis Magnetic focusing Hydrodynamic fractionation Biomedical Engineering Biological and Medical Physics, Biophysics Nanotechnology Engineering Fluid Dynamics
Content Type	Text
Resource Type	Article
Subject	Nanoscience and Nanotechnology Molecular Biology Biomedical Engineering

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1	Ministry of Education (GoI), Department of Higher Education	Sanctioning Authority	https://www.education.gov.in/ict-initiatives
2	Indian Institute of Technology Kharagpur	Host Institute of the Project: The host institute of the project is responsible for providing infrastructure support and hosting the project	https://www.iitkgp.ac.in
3	National Digital Library of India Office, Indian Institute of Technology Kharagpur	The administrative and infrastructural headquarters of the project	Dr. B. Sutradhar bsutra@ndl.gov.in
4	Project PI / Joint PI	Principal Investigator and Joint Principal Investigators of the project	Dr. B. Sutradhar bsutra@ndl.gov.in Prof. Saswat Chakrabarti will be added soon
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