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Binding of Euplotes Octocarinatus centrin with target peptide melittin
| Content Provider | Semantic Scholar |
|---|---|
| Author | Yaqin, Zhao Jiuying, Feng Aihua, Liang Binsheng, Yang |
| Copyright Year | 2007 |
| Abstract | Interactions between model target peptide melittin (ME) and Euplotes octocarinatus centrin (EoCen) were investigated by fluorescence spectra, circular dichroism (CD) spectra and native polyacrylamide gel electrophoresis (PAGE). In 0.1 mol/L N-2-hydroxyethylpiperazine-N-2-ethanesufonic acid (Hepes) and 150 mmol/L NaCl at pH 7.4, EoCen and isolated short C-terminal domain of EoCen (SC-EoCen) form 1:1 peptide:protein complexes. However, no detectable signal changes can be observed while isolated N-terminal domain of EoCen (N-EoCen) or isolated long C-terminal domain of EoCen (LC-EoCen) was added into solution of ME. The interaction between EoCen and ME is specified exclusively for the short C-terminal domain of EoCen. On the basis of fluorescence titration curves, the conditional binding constants of ME with EoCen and SC-EoCen were calculated to be logKME-EoCen = 6.81±0.33 and logKME-SC-EoCen = 6.51±0.45, respectively. |
| File Format | PDF HTM / HTML |
| Alternate Webpage(s) | http://ims.sxu.edu.cn/docs/20100612164406944461.pdf |
| Language | English |
| Access Restriction | Open |
| Content Type | Text |
| Resource Type | Article |
