NDLI: Optimization of adenosine 5′-triphosphate extraction for the measurement of␣acidogenic biomass utilizing whey wastewater

Content Provider	Springer Nature Link
Author	Lee, Changsoo Kim, Jaai Hwang, Seokhwan
Copyright Year	2005
Abstract	A set of experiments was carried out to maximize adenosine 5′-triphosphate (ATP) extraction efficiency from acidogenic culture using whey wastewater. ATP concentrations at different microbial concentrations increased linearly as microbial concentration decreased. More than 50% of ATP was extracted from the sample of 39 mg volatile suspended solids (VSS)/l compared to the sample of 2.8 g VSS/l. The ATP concentrations of the corresponding samples were 0.74±0.06 and 0.49±0.05 mg/l, respectively. For low VSS concentrations ranging from 39 to 92 mg/l, the extracted ATP concentration did not vary significantly at 0.73±0.01 mg ATP/l. Response surface methodology with a central composite in cube design for the experiments was used to locate the optimum for maximal ATP extraction with respect to boiling and bead beating treatments. The overall designed intervals were from 0 to 15 min and from 0 to 3 min for boiling and bead beating, respectively. The extracted ATP concentration ranged from 0.01 to 0.74 mg/l within the design boundary. The following is a partial cubic model where η is the concentration of ATP and x k is the corresponding variable term (k=boiling time and bead beating time in order): η=0.629+0.035x 1–0.818x 2–0.002x 1 x 2–0.003x 1 2 +0.254x 2 2 +0.002x 1 2 x 2. This model successfully approximates the response of ATP concentration with respect to the boiling- and bead beating-time. The condition for maximal ATP extraction was 5.6 min boiling without bead beating. The maximal ATP concentration using the model was 0.74 mg/l, which was identical to the experimental value at optimum condition for ATP extraction.
Starting Page	347
Ending Page	355
Page Count	9
File Format	PDF
ISSN	09239820
Journal	Biodegradation
Volume Number	17
Issue Number	4
e-ISSN	15729729
Language	English
Publisher	Kluwer Academic Publishers
Publisher Date	2006-02-21
Publisher Place	Dordrecht
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	acidogens; ATP extraction; bioluminescence; biomass; response surface methodology Waste Water Technology / Water Pollution Control / Water Management / Aquatic Pollution Waste Management/Waste Technology Terrestrial Pollution Soil Science & Conservation Geochemistry
Content Type	Text
Resource Type	Article
Subject	Environmental Chemistry Pollution Environmental Engineering Bioengineering Microbiology

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