NDLI: Correlation of microbial mass with ATP and DNA concentrations in acidogenesis of whey permeate

Content Provider	Springer Nature Link
Author	Kim, Jaai Lee, Changsoo Shin, Seung Gu Hwang, Seokhwan
Copyright Year	2007
Abstract	In this paper, we examine variations in the contents of ATP and DNA per unit microbial mass in an acidogenesis of whey permeate. We also introduce a novel approach to estimate microbial mass by measuring ATP and DNA when the ratios of ATP and DNA to microbial mass vary. Acidogenic experiments were performed at 35°C and pH 6.0 in batch mode. The amounts of ATP and DNA per unit microbial mass were not consistent during the incubation except during the post-decay phase. Especially within the exponential phase, each showed a 10-fold difference between maximal and minimal values. In this case, the conventional method which converts ATP or DNA concentration into microbial mass using a fixed conversion factor can give inaccurate results. While the constant ratios of 0.74 mg ATP/g VSS and 1.96 mg DNA/g VSS were determined for the post-decay phase, the ATP and DNA concentrations showed strong linear relationships with the microbial mass (r 2 = 0.99) within the ranges of 0.039–1.078 mg ATP/l and 0.075–2.080 mg DNA/l, respectively. The linear regression equations are as follows: (1) microbial mass concentration (mg/l) = 478.5 × ATP concentration (mg/l) + 293.5, (2) microbial mass concentration (mg/l) = 257.2 × DNA concentration (mg/l) + 250.4. Therefore, changes in the mass of the acidogenic population should be monitored by the combined use of the regression equations obtained in the exponential phase and the constant ratios determined in the post-decay phase. This procedure should be widely applicable to the acidogenesis of dairy processing wastewaters, especially of a highly suspended organic wastewater such as whey.
Starting Page	187
Ending Page	195
Page Count	9
File Format	PDF
ISSN	09239820
Journal	Biodegradation
Volume Number	19
Issue Number	2
e-ISSN	15729729
Language	English
Publisher	Springer Netherlands
Publisher Date	2007-05-09
Publisher Place	Dordrecht
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Acidogenesis ATP Dairy processing wastewater DNA Microbial mass Whey permeate Waste Management/Waste Technology Waste Water Technology / Water Pollution Control / Water Management / Aquatic Pollution Terrestrial Pollution Geochemistry Soil Science & Conservation
Content Type	Text
Resource Type	Article
Subject	Environmental Chemistry Pollution Environmental Engineering Bioengineering Microbiology

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