NDLI: Heterogeneous response to biostimulation for U(VI) reduction in replicated sediment microcosms

Content Provider	Springer Nature Link
Author	Nyman, Jennifer L. Marsh, Terence L. Ginder Vogel, Matthew A. Gentile, Margaret Fendorf, Scott Criddle, Craig
Copyright Year	2006
Abstract	A field-scale experiment to assess biostimulation of uranium reduction is underway at the Natural and Accelerated Bioremediation Research Field Research Center (FRC) in Oak Ridge, Tennessee. To simulate the field experiment, we established replicate batch microcosms containing well-mixed contaminated sediment from a well within the FRC treatment zone, and we added an inoculum from a pilot-scale fluidized bed reactor representing the inoculum in the field experiment. After reduction of nitrate, both sulfate and soluble U(VI) concentration decreased. X-ray absorption near edge structure (XANES) spectroscopy confirmed formation of U(IV) in sediment from biostimulated microcosms, but did not detect reduction of solid-phase Fe(III). Two to three fragments dominated terminal restriction fragment length polymorphism (T-RFLP) profiles of the 16S rDNA gene. Comparison to a clone library indicated these fragments represented denitrifying organisms related to Acidovorax, and Acidovorax isolates from the inoculum were subsequently shown to reduce U(VI). Investigation using the T-RFLP Analysis Program (TAP T-RFLP) and chemical analyses detected the presence and activity of fermenting and sulfate-reducing bacteria after 2 weeks. These organisms likely contributed to uranium reduction. In some microcosms, soluble U(VI) concentration leveled off or rebounded, indicating microbial and/or mineralogical heterogeneity among samples. Sulfate, acetate, and ethanol were depleted only in those microcosms exhibiting a rebound in soluble U(VI). This suggests that rates of U(VI) desorption can exceed rates of U(VI) reduction when sulfate-reducing bacteria become substrate-limited. These observations underscore the importance of effective chemical delivery and the role of serial and parallel processes in uranium reduction.
Starting Page	303
Ending Page	316
Page Count	14
File Format	PDF
ISSN	09239820
Journal	Biodegradation
Volume Number	17
Issue Number	4
e-ISSN	15729729
Language	English
Publisher	Kluwer Academic Publishers
Publisher Date	2006-02-21
Publisher Place	Dordrecht
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	16S rDNA bioremediation subsurface sulfate-reducing bacteria T-RFLP uranium reduction Waste Water Technology / Water Pollution Control / Water Management / Aquatic Pollution Waste Management/Waste Technology Terrestrial Pollution Soil Science & Conservation Geochemistry
Content Type	Text
Resource Type	Article
Subject	Environmental Chemistry Pollution Environmental Engineering Bioengineering Microbiology

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