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| Content Provider | Springer Nature Link |
|---|---|
| Author | Gozlan, S. Millot, P. Rousset, A. Fournier, D. |
| Copyright Year | 1997 |
| Abstract | Bugs from the genusOrius (Heteroptera, Anthocoridae) are used as biological control predators against acari and homopteran pests. This augmentative biological control method consists of massive field releases where ever indigenous populations may be present. Identification of the released predators along with the indigenous ones is a prerequisite to estimate the efficiency of the biological control agent. We have tested whether strain-specific molecular markers can be used to discriminate strains of predators. The RAPD-PCR method was used. Species are easily distinguishable by this technique, but, differentiation of strains from distinct geographic origins was impossible due to the high level of polymorphism observed. Reduction of this polymorphism by inbreeding lines and selection of rare alleles may allow to overcome this problem.Les punaises du genreOrius (Het. : Anthocoridae) sont utilisées en lutte biologique comme prédateurs d’acariens et d’homoptères ravageurs. Cette méthode de lutte biologique par renforcement consiste en des lâchers de masse au champ où d’éventuelles populations indigènes du même prédateur peuvent être déjà présentes. La possibilité de distinguer les prédateurs lâchés de ceux indigènes est la condition préalable à une bonne estimation de l’efficacité de l’agent de lutte biologique. Nous avons expérimenté la méthode RAPD-PCR pour voir si des marqueurs moléculaires pouvaient être utilisés pour distinguer les souches de prédateurs. Les espèces sont facilement distinguables par cette technique mais la différenciation des souches d’origines géographiques différentes a été impossible en raison d’un degré élevé de polymorphisme observé. La réduction de ce polymorphisme par des souches homozygotes et la sélection d’allèles rares pourrait permettre de surmonter ce problème. |
| Starting Page | 593 |
| Ending Page | 604 |
| Page Count | 12 |
| File Format | |
| ISSN | 00138959 |
| Journal | BioControl |
| Volume Number | 42 |
| Issue Number | 4 |
| e-ISSN | 15738248 |
| Language | French |
| Publisher | Springer Netherlands |
| Publisher Date | 1997-01-01 |
| Publisher Place | Dordrecht |
| Access Restriction | One Nation One Subscription (ONOS) |
| Subject Keyword | RAPD biological control polymorphism Entomology Plant Pathology Agriculture Animal Ecology Animal Biochemistry Behavioural Sciences |
| Content Type | Text |
| Resource Type | Article |
| Subject | Plant Science Ecology, Evolution, Behavior and Systematics Agricultural and Biological Sciences |
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