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| Content Provider | Springer Nature Link |
|---|---|
| Author | Shoibov, B. B. Grigor’eva, D. V. Shabalina, A. A. Kostyreva, M. V. Baronets, V. Yu. Zamolodchikova, T. S. |
| Copyright Year | 2015 |
| Abstract | We proposed a new indicator for evaluation of functional activity of Ca2+ in human blood serum based on lysis of sheep erythrocytes with 10% human blood serum in the presence of 0.55 mM ethylene glycol tetraacetic acid at 37оC for 10 min. After incubation, the degree of sheep erythrocytes lysis inhibition is estimated: inhibition of complement hemolytic activity <30% is considered as high functional Са2+ activity, inhibition by 31-70% corresponds to normal activity, and >71% indicates low activity. The comparative studies of complement activating function of heterophilic antibodies, complement system reactivity in the presence of 0.29 M NaCl, and functional activity of ionized Ca2+ make possible estimation of the individual’s immune status. |
| Starting Page | 305 |
| Ending Page | 308 |
| Page Count | 4 |
| File Format | |
| ISSN | 00074888 |
| Journal | Bulletin of Experimental Biology and Medicine |
| Volume Number | 159 |
| Issue Number | 3 |
| e-ISSN | 15738221 |
| Language | English |
| Publisher | Springer US |
| Publisher Date | 2015-07-25 |
| Publisher Place | New York |
| Access Restriction | One Nation One Subscription (ONOS) |
| Subject Keyword | complement system ethylene glycol tetraacetic acid functional activity of ionized Ca2+ complement inhibition Biomedicine general Internal Medicine Cell Biology Pathology Laboratory Medicine |
| Content Type | Text |
| Resource Type | Article |
| Subject | Medicine Biochemistry, Genetics and Molecular Biology |
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