NDLI: Anisotropic Nature of Mouse Lung Parenchyma

Content Provider	Springer Nature Link
Author	Mitzner, Wayne Fallica, Jonathan Bishai, John
Copyright Year	2008
Abstract	Lung parenchyma is normally considered to be isotropic, that is, its properties do not depend upon specific preferential directions. The assumption of isotropy is important for both modeling of lung mechanical properties and quantitative histologic measurements. This assumption, however, has not been previously examined at the microscopic level, in part because of the difficulty in large lungs of obtaining sufficient numbers of small samples of tissue while maintaining the spatial orientation. In the mouse, however, this difficulty is minimized. We evaluated the parenchymal isotropy in mouse lungs by quantifying the mean airspace chord lengths (Lm) from high-resolution histology of complete sections surrounded by an intact continuous visceral pleural membrane. We partitioned this lung into 5 isolated regions, defined by the distance from the visceral pleura. To further evaluate the isotropy, we also measured Lm in two orthogonal spatial directions with respect to the section orientation, and varied the sample line spacing from 3 to 280 μm. Results show a striking degree of parenchymal anisotropy in normal mouse lungs. The Lm was significantly greater when grid lines were parallel to the ventral–dorsal axis of the tissue. In addition the Lm was significantly smaller within 300 μm of the visceral pleura. Whether this anisotropy results from intrinsic structural factors or from nonuniform shrinkage during conventional tissue processing is uncertain, but it should be considered when interpreting quantitative morphometric measurements made in the mouse lung.
Starting Page	2111
Ending Page	2120
Page Count	10
File Format	PDF
ISSN	00906964
Journal	Annals of Biomedical Engineering
Volume Number	36
Issue Number	12
e-ISSN	15739686
Language	English
Publisher	Springer US
Publisher Date	2008-07-17
Publisher Place	Boston
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Morphometry Histology Alveolar structure Heterogeneity Stereology Isotropy Biochemistry Mechanics Biophysics/Biomedical Physics Biomedical Engineering Biomedicine general
Content Type	Text
Resource Type	Article
Subject	Biomedical Engineering

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