NDLI: Co-Immobilization of Gradient-Patterned Growth Factors for Directed Cell Migration

Content Provider	Springer Nature Link
Author	Stefonek Puccinelli, Tracy Jane Masters, Kristyn S.
Copyright Year	2008
Abstract	Cell migration is critically important for the repair of chronic wounds, which cost billions of dollars each year to treat and can lead to serious complications, including amputation and death. Growth factors, including epidermal growth factor (EGF) and insulin-like growth factor-1 (IGF-1), are known to be deficient in chronic wounds; unfortunately, traditional delivery of soluble growth factors to wounds is expensive and complicated by their degradation. We have previously shown that directed and accelerated keratinocyte migration could be achieved by creating immobilized gradients of EGF. In this work, we have optimized EGF gradients for cell migration, synthesized and characterized gradient patterns of IGF-1, and tested for migration synergy upon combination of EGF and IGF-1 patterns. An optimal EGF concentration and pattern was identified, resulting in migration that was almost 10-fold that achieved on unpatterned controls. Immobilization of IGF-1 gradients also accelerated and directed keratinocyte migration (p < 0.05), however, no difference in migration was found across various IGF-1 concentrations or gradient patterns. Although combining EGF with IGF-1 patterns did not accelerate migration beyond levels achieved using EGF alone, these methods can be applied to create other types of multi-component gradients that may ultimately be utilized to create bioactive wound dressings.
Starting Page	2121
Ending Page	2133
Page Count	13
File Format	PDF
ISSN	00906964
Journal	Annals of Biomedical Engineering
Volume Number	36
Issue Number	12
e-ISSN	15739686
Language	English
Publisher	Springer US
Publisher Date	2008-10-11
Publisher Place	Boston
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Biomolecule tethering Epidermal growth factor Insulin-like growth factor-1 Keratinocytes Wound healing Biochemistry Mechanics Biophysics/Biomedical Physics Biomedical Engineering Biomedicine general
Content Type	Text
Resource Type	Article
Subject	Biomedical Engineering

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