NDLI: Fluid Flow-Induced Calcium Response in Early or Late Differentiated Osteoclasts

Content Provider	Springer Nature Link
Author	Li, Ping Hu, Man Sun, Shujin Zhang, Yan Gao, Yuxin Long, Mian Huo, Bo Zhang, Ding
Copyright Year	2012
Abstract	Intracellular calcium oscillation caused by receptor activator of nuclear factor kappa-B ligand has been demonstrated to promote the differentiation of osteoclasts. Osteoclasts are recruited on the surface of trabeculae, and are exposed to fluid flow caused by the deformation of the bone matrix. However, the roles of fluid shear stress (FSS) on calcium response during the differentiation process of osteoclasts are still unknown. In the current study, the formation of tartrate-resistant acid phosphatase-positive, multinucleated osteoclasts from RAW264.7 macrophage cells were induced by co-culturing them with the conditioned medium from MC3T3-E1 osteoblasts. The in situ observations showed a high correlation between the area and the nuclear number of osteoclasts. The cells were stimulated by FSS at different levels (1 or 10 dyne/cm2) before (0 day) or after being induced for 4 or 8 days. The mechanically-induced calcium response was recorded and analyzed. The results indicated a different property of calcium oscillation for the osteoclasts in different fusion stages (i.e., more calcium-responsive peaks appeared in small osteoclasts than those in the larger ones). The rates of calcium influx decreased and the time of recovery in osteoclast cytosol increased along with the fusion of osteoclasts. In addition, increasing the FSS level enhanced the calcium oscillation of osteoclasts at early induction (4 days). However, this effect was weakened at the late induction (8 days). The present work could help provide understanding regarding the mechanism of the involvement of calcium in mechanically induced bone remodeling.
Starting Page	1874
Ending Page	1883
Page Count	10
File Format	PDF
ISSN	00906964
Journal	Annals of Biomedical Engineering
Volume Number	40
Issue Number	9
e-ISSN	15739686
Language	English
Publisher	Springer US
Publisher Date	2012-04-25
Publisher Place	Boston
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Osteoclasts Fluid shear stress Calcium oscillation Induction time Differentiation Mechanics Biophysics and Biological Physics Biochemistry Biomedical Engineering Biomedicine general
Content Type	Text
Resource Type	Article
Subject	Biomedical Engineering

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