NDLI: Ex Vivo Gene Delivery to Hepatocytes: Techniques, Challenges, and Underlying Mechanisms

Content Provider	Springer Nature Link
Author	Gao, Shan Seker, Erkin Casali, Monica Wang, Fangjing Bale, Shyam Sundhar Price, Gavrielle M. Yarmush, Martin L.
Copyright Year	2012
Abstract	Gene delivery to primary hepatocytes is an important tool for a number of applications including the study of liver cell biology and pathology, drug screening, and gene therapy. Robust transfection of primary hepatocytes, however, is significantly more difficult to achieve than in cell lines or readily dividing primary cells. In this report, we investigated in vitro gene delivery to both primary rat hepatocytes and Huh7.5.1 cells (a hepatoma cell line) using a number of viral and non-viral methods, including Lipofectamine 2000, FuGene HD, Nucleofection, Magnetofection, and lentiviruses. Our results showed that Lipofectamine 2000 is the most efficient reagent for green fluorescent protein (GFP) gene delivery to primary rat hepatocytes (33.3 ± 1.8% transfection efficiency) with minimal adverse effect on several hepatic functions, such as urea and albumin secretion. The lentiviral vectors used in this study exhibited undetectable gene delivery to primary rat hepatocytes but significant delivery to Huh7.5.1 cells (>80% transfection efficiency). In addition, we demonstrated lentiviral-based and spatially defined delivery of the GFP gene to Huh7.5.1 cells for use in biological microelectromechanical systems.
Starting Page	1851
Ending Page	1861
Page Count	11
File Format	PDF
ISSN	00906964
Journal	Annals of Biomedical Engineering
Volume Number	40
Issue Number	9
e-ISSN	15739686
Language	English
Publisher	Springer US
Publisher Date	2012-04-07
Publisher Place	Boston
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Non-viral transfection Lentiviruses rat hepatocytes Huh7.5.1 Transfection efficiency Hepatic function Mechanics Biophysics and Biological Physics Biochemistry Biomedical Engineering Biomedicine general
Content Type	Text
Resource Type	Article
Subject	Biomedical Engineering

Sl.	Authority	Responsibilities	Communication Details
1	Ministry of Education (GoI), Department of Higher Education	Sanctioning Authority	https://www.education.gov.in/ict-initiatives
2	Indian Institute of Technology Kharagpur	Host Institute of the Project: The host institute of the project is responsible for providing infrastructure support and hosting the project	https://www.iitkgp.ac.in
3	National Digital Library of India Office, Indian Institute of Technology Kharagpur	The administrative and infrastructural headquarters of the project	Dr. B. Sutradhar bsutra@ndl.gov.in
4	Project PI / Joint PI	Principal Investigator and Joint Principal Investigators of the project	Dr. B. Sutradhar bsutra@ndl.gov.in Prof. Saswat Chakrabarti will be added soon
5	Website/Portal (Helpdesk)	Queries regarding NDLI and its services	support@ndl.gov.in
6	Contents and Copyright Issues	Queries related to content curation and copyright issues	content@ndl.gov.in
7	National Digital Library of India Club (NDLI Club)	Queries related to NDLI Club formation, support, user awareness program, seminar/symposium, collaboration, social media, promotion, and outreach	clubsupport@ndl.gov.in
8	Digital Preservation Centre (DPC)	Assistance with digitizing and archiving copyright-free printed books	dpc@ndl.gov.in
9	IDR Setup or Support	Queries related to establishment and support of Institutional Digital Repository (IDR) and IDR workshops	idr@ndl.gov.in

Ex Vivo Gene Delivery to Hepatocytes: Techniques, Challenges, and Underlying Mechanisms

Physical Non-Viral Gene Delivery Methods for Tissue Engineering

Laser-Assisted Cryosurgery in ex vivo Mice Hepatic Tissue: Viability Assays Using Green Fluorescent Protein

Voxelized Computational Model for Convection-Enhanced Delivery in the Rat Ventral Hippocampus: Comparison with In Vivo MR Experimental Studies

Applicator for RF Thermokeratoplasty: Feasibility Study Using Theoretical Modeling and Ex Vivo Experiments

In Vivo Contrast-Enhanced MR Imaging of Direct Infusion into Rat Peripheral Nerves

2011 Rita Schaffer Lecture: Nanoparticles for Intracellular Nucleic Acid Delivery

Delivery of Viral Vectors to Tumor Cells: Extracellular Transport, Systemic Distribution, and Strategies for Improvement

Biomolecule Delivery to Engineer the Cellular Microenvironment for Regenerative Medicine

Ex Vivo Gene Delivery to Hepatocytes: Techniques, Challenges, and Underlying Mechanisms

Similar Documents

Ex Vivo Gene Delivery to Hepatocytes: Techniques, Challenges, and Underlying Mechanisms

Physical Non-Viral Gene Delivery Methods for Tissue Engineering

Laser-Assisted Cryosurgery in ex vivo Mice Hepatic Tissue: Viability Assays Using Green Fluorescent Protein

Voxelized Computational Model for Convection-Enhanced Delivery in the Rat Ventral Hippocampus: Comparison with In Vivo MR Experimental Studies

Applicator for RF Thermokeratoplasty: Feasibility Study Using Theoretical Modeling and Ex Vivo Experiments

In Vivo Contrast-Enhanced MR Imaging of Direct Infusion into Rat Peripheral Nerves

2011 Rita Schaffer Lecture: Nanoparticles for Intracellular Nucleic Acid Delivery

Delivery of Viral Vectors to Tumor Cells: Extracellular Transport, Systemic Distribution, and Strategies for Improvement

Biomolecule Delivery to Engineer the Cellular Microenvironment for Regenerative Medicine

Ex Vivo Gene Delivery to Hepatocytes: Techniques, Challenges, and Underlying Mechanisms