NDLI: Low-Intensity Pulsed Ultrasound Modulates Shear Stress Induced PGHS-2 Expression and PGE2 Synthesis in MLO-Y4 Osteocyte-Like Cells

Content Provider	Springer Nature Link
Author	Saini, Vikas Yadav, Sumita McCormick, Susan
Copyright Year	2010
Abstract	Fluid shear stress (SS) has been shown to be a prevailing physiological stimulus in the regulation of bone cell metabolism and so are the exogenous biomechanical forces, like ultrasound (US) and vibration. The purpose of this study is to elaborate the interplay of laminar fluid SS with low-intensity pulsed US in the regulation of prostaglandin H synthase 2 (PGHS-2) and prostaglandin E2 (PGE2). Murine long bone osteocyte-like (MLO-Y4) cells were exposed to various regimes of US (1.5 Hz, 30 mW/cm2) and SS (19 dyn/cm2) alone and sequentially. Changes in PGHS-2 gene expression levels were quantified at 3 and 24 h using real-time RT-PCR. PGE2 levels in the culture media were measured using enzyme immunoassay at 3 and 24 h. PGE2 levels significantly increased after exposure to SS for 3 and 24 h by 2.17 ± 0.02 and 5.47 ± 0.42-fold, respectively, compared to control cells. A 20 min US treatment prior to SS significantly increased SS PGE2 levels 2.95 ± 0.18 and 2.90 ± 0.50-fold at 3 and 24 h, respectively. US also significantly increased PGHS-2 mRNA levels in cells exposed to SS. SS caused a 2.74 ± 0.49-fold increase in PGHS-2 mRNA levels at 3 h and a significant 3.70 ± 0.25-fold increase at 24 h relative to control. A 20 min US treatment caused 1.35 ± 0.49 and 2.44 ± 0.82-fold increase in PGHS-2 mRNA levels in cells exposed to SS at 3 and 24 h, respectively. These results indicate that combining US with SS may have a more anabolic benefit for bone tissue than either stimulus alone.
Starting Page	378
Ending Page	393
Page Count	16
File Format	PDF
ISSN	00906964
Journal	Annals of Biomedical Engineering
Volume Number	39
Issue Number	1
e-ISSN	15739686
Language	English
Publisher	Springer US
Publisher Date	2010-09-04
Publisher Place	Boston
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Biomechanical force PGHS-1 PGHS-2 MLO-Y4 osteocyte-like cells Biochemistry Mechanics Biophysics and Biological Physics Biomedical Engineering Biomedicine general
Content Type	Text
Resource Type	Article
Subject	Biomedical Engineering

Sl.	Authority	Responsibilities	Communication Details
1	Ministry of Education (GoI), Department of Higher Education	Sanctioning Authority	https://www.education.gov.in/ict-initiatives
2	Indian Institute of Technology Kharagpur	Host Institute of the Project: The host institute of the project is responsible for providing infrastructure support and hosting the project	https://www.iitkgp.ac.in
3	National Digital Library of India Office, Indian Institute of Technology Kharagpur	The administrative and infrastructural headquarters of the project	Dr. B. Sutradhar bsutra@ndl.gov.in
4	Project PI / Joint PI	Principal Investigator and Joint Principal Investigators of the project	Dr. B. Sutradhar bsutra@ndl.gov.in Prof. Saswat Chakrabarti will be added soon
5	Website/Portal (Helpdesk)	Queries regarding NDLI and its services	support@ndl.gov.in
6	Contents and Copyright Issues	Queries related to content curation and copyright issues	content@ndl.gov.in
7	National Digital Library of India Club (NDLI Club)	Queries related to NDLI Club formation, support, user awareness program, seminar/symposium, collaboration, social media, promotion, and outreach	clubsupport@ndl.gov.in
8	Digital Preservation Centre (DPC)	Assistance with digitizing and archiving copyright-free printed books	dpc@ndl.gov.in
9	IDR Setup or Support	Queries related to establishment and support of Institutional Digital Repository (IDR) and IDR workshops	idr@ndl.gov.in

Changes in NO, iNOS and eNOS Expression in MLO-Y4 Cells After Low-intensity Pulsed Ultrasound Treatment With or Without Shear Stress Exposure

Effects of Low-Intensity Pulsed Ultrasound on the Expression and Activity of Hyaluronan Synthase and Hyaluronidase in IL-1β-Stimulated Synovial Cells

Low-Intensity Pulsed Ultrasound Activates Integrin-Mediated Mechanotransduction Pathway in Synovial Cells

Endothelial Insulin-Like Growth Factor-1 Modulates Proliferation and Phenotype of Smooth Muscle Cells Induced by Low Shear Stress

Mechanical Stimulation of Gap Junctions in Bone Osteocytes is Mediated by Prostaglandin E2

Mechanical behavior of an individual adherent MLO-Y4 osteocyte under shear flow

The Impact of the Extracellular Matrix Environment on Sost Expression by the MLO-Y4 Osteocyte Cell Line

Low-Intensity Pulsed Ultrasound in Dentofacial Tissue Engineering

Shear Stress Differentially Regulates PGHS-1 and PGHS-2 Protein Levels in Human Endothelial Cells

Low-Intensity Pulsed Ultrasound Modulates Shear Stress Induced PGHS-2 Expression and PGE2 Synthesis in MLO-Y4 Osteocyte-Like Cells

Similar Documents

Changes in NO, iNOS and eNOS Expression in MLO-Y4 Cells After Low-intensity Pulsed Ultrasound Treatment With or Without Shear Stress Exposure

Effects of Low-Intensity Pulsed Ultrasound on the Expression and Activity of Hyaluronan Synthase and Hyaluronidase in IL-1β-Stimulated Synovial Cells

Low-Intensity Pulsed Ultrasound Activates Integrin-Mediated Mechanotransduction Pathway in Synovial Cells

Endothelial Insulin-Like Growth Factor-1 Modulates Proliferation and Phenotype of Smooth Muscle Cells Induced by Low Shear Stress

Mechanical Stimulation of Gap Junctions in Bone Osteocytes is Mediated by Prostaglandin E2

Mechanical behavior of an individual adherent MLO-Y4 osteocyte under shear flow

The Impact of the Extracellular Matrix Environment on Sost Expression by the MLO-Y4 Osteocyte Cell Line

Low-Intensity Pulsed Ultrasound in Dentofacial Tissue Engineering

Shear Stress Differentially Regulates PGHS-1 and PGHS-2 Protein Levels in Human Endothelial Cells

Low-Intensity Pulsed Ultrasound Modulates Shear Stress Induced PGHS-2 Expression and PGE2 Synthesis in MLO-Y4 Osteocyte-Like Cells