NDLI: Fusion of Concentrically Layered Tubular Tissue Constructs Increases Burst Strength

Content Provider	Springer Nature Link
Author	Huynh, Thanh N. Tranquillo, Robert T.
Copyright Year	2010
Abstract	Tubular tissue constructs prepared from neonatal human dermal fibroblasts entrapped in fibrin gel were incubated on a mandrel for three weeks to allow for initial fibrin remodeling into tissue before being concentrically layered and incubated for an additional three weeks on the mandrel. Upon harvest, double layer constructs were not statistically different from single layer control constructs in terms of length, collagen density, cell density, tensile modulus, or ultimate tensile strength. However, the thickness and burst pressure were both approximately twice the single layer control values. Metabolically active cells were detected at the interface, and scanning electron microscopy revealed fiber structures bridging the two layers, co-localizing with the cells, which exhibited minimal migration across the layers. In contrast, double layer constructs where tissue fusion was prohibited by mechanical distraction of the layers showed no increase in burst pressure despite having increased thickness and the same collagen and cell densities of the single layer control constructs; moreover, the burst failure occurred sequentially in the layers in contrast to simultaneous failure for the fused double layer constructs. This study provides insight into the nature of the interface and the role of cell behavior when tissue fusion occurs between two layers of bioartificial tissue in vitro. It also suggests a method for improving the burst strength of fibrin-based tubular tissue constructs by increasing the construct thickness via concentrically layering and fusing two constructs.
Starting Page	2226
Ending Page	2236
Page Count	11
File Format	PDF
ISSN	00906964
Journal	Annals of Biomedical Engineering
Volume Number	38
Issue Number	6
e-ISSN	15739686
Language	English
Publisher	Springer US
Publisher Date	2010-04-30
Publisher Place	Boston
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Tissue engineering Vascular engineering Tissue-engineered vascular graft Tissue-engineered blood vessel Tissue fusion Biochemistry Mechanics Biophysics and Biological Physics Biomedical Engineering Biomedicine general
Content Type	Text
Resource Type	Article
Subject	Biomedical Engineering

Sl.	Authority	Responsibilities	Communication Details
1	Ministry of Education (GoI), Department of Higher Education	Sanctioning Authority	https://www.education.gov.in/ict-initiatives
2	Indian Institute of Technology Kharagpur	Host Institute of the Project: The host institute of the project is responsible for providing infrastructure support and hosting the project	https://www.iitkgp.ac.in
3	National Digital Library of India Office, Indian Institute of Technology Kharagpur	The administrative and infrastructural headquarters of the project	Dr. B. Sutradhar bsutra@ndl.gov.in
4	Project PI / Joint PI	Principal Investigator and Joint Principal Investigators of the project	Dr. B. Sutradhar bsutra@ndl.gov.in Prof. Saswat Chakrabarti will be added soon
5	Website/Portal (Helpdesk)	Queries regarding NDLI and its services	support@ndl.gov.in
6	Contents and Copyright Issues	Queries related to content curation and copyright issues	content@ndl.gov.in
7	National Digital Library of India Club (NDLI Club)	Queries related to NDLI Club formation, support, user awareness program, seminar/symposium, collaboration, social media, promotion, and outreach	clubsupport@ndl.gov.in
8	Digital Preservation Centre (DPC)	Assistance with digitizing and archiving copyright-free printed books	dpc@ndl.gov.in
9	IDR Setup or Support	Queries related to establishment and support of Institutional Digital Repository (IDR) and IDR workshops	idr@ndl.gov.in

Pulsatile Perfusion Bioreactor System for Durability Testing and Compliance Estimation of Tissue Engineered Vascular Grafts

Tubular Heart Valves from Decellularized Engineered Tissue

Cellularized Microcarriers as Adhesive Building Blocks for Fabrication of Tubular Tissue Constructs

Effect of Strain Magnitude on the Tissue Properties of Engineered Cardiovascular Constructs

Approach for Fabricating Tissue Engineered Vascular Grafts with Stable Endothelialization

Tissue Engineered Constructs: Perspectives on Clinical Translation

Vascularization in Bone Tissue Engineering Constructs

Sequential Multimodal Microscopic Imaging and Biaxial Mechanical Testing of Living Multicomponent Tissue Constructs

3D Printing of Tissue Engineered Constructs for In Vitro Modeling of Disease Progression and Drug Screening

Fusion of Concentrically Layered Tubular Tissue Constructs Increases Burst Strength

Similar Documents

Pulsatile Perfusion Bioreactor System for Durability Testing and Compliance Estimation of Tissue Engineered Vascular Grafts

Tubular Heart Valves from Decellularized Engineered Tissue

Cellularized Microcarriers as Adhesive Building Blocks for Fabrication of Tubular Tissue Constructs

Effect of Strain Magnitude on the Tissue Properties of Engineered Cardiovascular Constructs

Approach for Fabricating Tissue Engineered Vascular Grafts with Stable Endothelialization

Tissue Engineered Constructs: Perspectives on Clinical Translation

Vascularization in Bone Tissue Engineering Constructs

Sequential Multimodal Microscopic Imaging and Biaxial Mechanical Testing of Living Multicomponent Tissue Constructs

3D Printing of Tissue Engineered Constructs for In Vitro Modeling of Disease Progression and Drug Screening

Fusion of Concentrically Layered Tubular Tissue Constructs Increases Burst Strength