NDLI: Sequential Multimodal Microscopic Imaging and Biaxial Mechanical Testing of Living Multicomponent Tissue Constructs

Content Provider	Springer Nature Link
Author	Bai, Yuqiang Lee, Po Feng Humphrey, Jay D. Yeh, Alvin T.
Copyright Year	2014
Abstract	Understanding relationships between mechanical stimuli and cellular responses require measurements of evolving tissue structure and mechanical properties. We developed a 3D tissue bioreactor that couples to both the stage of a custom multimodal microscopy system and a biaxial mechanical testing platform. Time dependent changes in microstructure and mechanical properties of fibroblast seeded cruciform fibrin gels were investigated while cultured under either anchored (1.0:1.0 stretch ratio) or strip biaxial (1.0:1.1) conditions. A multimodal nonlinear optical microscopy-optical coherence microscopy (NLOM-OCM) system was used to delineate noninvasively the relative spatial distributions of original fibrin, deposited collagen, and fibroblasts during month long culture. Serial in-culture mechanical testing was also performed to track the evolution of bulk mechanical properties under sterile conditions. Over the month long time course, seeded cells and deposited collagen were randomly distributed in equibiaxially anchored constructs, but exhibited preferential alignment parallel to the direction of the 10% stretch in constructs cultured under strip biaxial stretch. Surprisingly, both anchored and strip biaxial stretched constructs exhibited isotropic mechanical properties (including progressively increasing stiffness) despite developing a very different collagen microstructural organization. In summary, our biaxial bioreactor system integrating both NLOM-OCM and mechanical testing provided complementary information on microstructural organization and mechanical properties and, thus, may enable greater fundamental understanding of relationships between engineered soft tissue mechanics and mechanobiology.
Starting Page	1791
Ending Page	1805
Page Count	15
File Format	PDF
ISSN	00906964
Journal	Annals of Biomedical Engineering
Volume Number	42
Issue Number	9
e-ISSN	15739686
Language	English
Publisher	Springer US
Publisher Date	2014-05-10
Publisher Place	Boston
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Biomechanics NLOM-OCM imaging Fibrin Engineered tissue Biomedicine general Biomedical Engineering Biophysics and Biological Physics Mechanics Biochemistry
Content Type	Text
Resource Type	Article
Subject	Biomedical Engineering

Sl.	Authority	Responsibilities	Communication Details
1	Ministry of Education (GoI), Department of Higher Education	Sanctioning Authority	https://www.education.gov.in/ict-initiatives
2	Indian Institute of Technology Kharagpur	Host Institute of the Project: The host institute of the project is responsible for providing infrastructure support and hosting the project	https://www.iitkgp.ac.in
3	National Digital Library of India Office, Indian Institute of Technology Kharagpur	The administrative and infrastructural headquarters of the project	Dr. B. Sutradhar bsutra@ndl.gov.in
4	Project PI / Joint PI	Principal Investigator and Joint Principal Investigators of the project	Dr. B. Sutradhar bsutra@ndl.gov.in Prof. Saswat Chakrabarti will be added soon
5	Website/Portal (Helpdesk)	Queries regarding NDLI and its services	support@ndl.gov.in
6	Contents and Copyright Issues	Queries related to content curation and copyright issues	content@ndl.gov.in
7	National Digital Library of India Club (NDLI Club)	Queries related to NDLI Club formation, support, user awareness program, seminar/symposium, collaboration, social media, promotion, and outreach	clubsupport@ndl.gov.in
8	Digital Preservation Centre (DPC)	Assistance with digitizing and archiving copyright-free printed books	dpc@ndl.gov.in
9	IDR Setup or Support	Queries related to establishment and support of Institutional Digital Repository (IDR) and IDR workshops	idr@ndl.gov.in

Strain-induced Collagen Organization at the Micro-level in Fibrin-based Engineered Tissue Constructs

Remodeling of Engineered Tissue Anisotropy in Response to Altered Loading Conditions

The Effect of Intermittent Static Biaxial Tensile Strains on Tissue Engineered Cartilage

Fusion of Concentrically Layered Tubular Tissue Constructs Increases Burst Strength

Non-invasive and Non-destructive Characterization of Tissue Engineered Constructs Using Ultrasound Imaging Technologies: A Review

A Computer-Controlled Apparatus for the Characterization of Mechanical and Viscoelastic Properties of Tissue-Engineered Vascular Constructs

Tissue Engineered Constructs: Perspectives on Clinical Translation

Cyclic Stretch and Perfusion Bioreactor for Conditioning Large Diameter Engineered Tissue Tubes

Effect of Strain Magnitude on the Tissue Properties of Engineered Cardiovascular Constructs

Sequential Multimodal Microscopic Imaging and Biaxial Mechanical Testing of Living Multicomponent Tissue Constructs

Similar Documents

Strain-induced Collagen Organization at the Micro-level in Fibrin-based Engineered Tissue Constructs

Remodeling of Engineered Tissue Anisotropy in Response to Altered Loading Conditions

The Effect of Intermittent Static Biaxial Tensile Strains on Tissue Engineered Cartilage

Fusion of Concentrically Layered Tubular Tissue Constructs Increases Burst Strength

Non-invasive and Non-destructive Characterization of Tissue Engineered Constructs Using Ultrasound Imaging Technologies: A Review

A Computer-Controlled Apparatus for the Characterization of Mechanical and Viscoelastic Properties of Tissue-Engineered Vascular Constructs

Tissue Engineered Constructs: Perspectives on Clinical Translation

Cyclic Stretch and Perfusion Bioreactor for Conditioning Large Diameter Engineered Tissue Tubes

Effect of Strain Magnitude on the Tissue Properties of Engineered Cardiovascular Constructs

Sequential Multimodal Microscopic Imaging and Biaxial Mechanical Testing of Living Multicomponent Tissue Constructs