NDLI: Non-denaturing low-temperature bonding of patterned poly(methyl methacrylate) enzymatic microreactors

Content Provider	Springer Nature Link
Author	Douma, Michelle D. Brown, Laurie Koerner, Terry Hugh Horton, J. Oleschuk, Richard D.
Copyright Year	2012
Abstract	A low-temperature solvent bonding system using methanol and water has been developed to bond poly(methyl methacrylate) (PMMA) microchips at 35 °C. The substrate/cover plate adhesion strengths obtained with this bonding protocol peaked at 4,000 kN/m2 for unmodified PMMA substrates. Nanoindentation measurements performed using atomic force microscopy revealed that only the first 30 nm of the PMMA surface showed a decreased hardness following surface modification and solvent treatment of the PMMA surface allowing the channel architecture to be maintained. The low temperature utilized for bonding enabled both a temperature-robust and temperature-labile enzyme to be facilely patterned prior to bonding with little-to-no loss in enzyme activity. Furthermore, the bonding methodology could be customized and used to fabricate an enzyme microreactor with pepsin (a pH, temperature and solvent sensitive enzyme). The enzyme microreactor performance was characterized by the longevity of the microreactor, as well as the efficiency of the protein digest performed. Enzyme immobilized with WSC decreased over a period of days, whereas the enzyme immobilized with both WSC (water soluble carbodiimide and NHS (N-hydroxysuccinimide) remained active even after a month of use. Short 10 mm column lengths with limited residence time provided high protein sequence coverage.
Starting Page	133
Ending Page	143
Page Count	11
File Format	PDF
ISSN	16134982
Journal	Microfluidics and Nanofluidics
Volume Number	14
Issue Number	1-2
e-ISSN	16134990
Language	English
Publisher	Springer-Verlag
Publisher Date	2012-09-01
Publisher Place	Berlin, Heidelberg
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Microreactor Proteolytic digestion Low temperature bonding Polymemthylmethacrylate Solvent bonding Engineering Fluid Dynamics Biomedical Engineering Analytical Chemistry Nanotechnology and Microengineering
Content Type	Text
Resource Type	Article
Subject	Nanoscience and Nanotechnology Materials Chemistry Condensed Matter Physics Electronic, Optical and Magnetic Materials

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