NDLI: Microfabrication-free fused silica nanofluidic interface for on chip electrokinetic stacking of DNA

Content Provider	Springer Nature Link
Author	Zhang, Da Wei Zhang, Hui Qin Tian, Li Wang, Lin Fang, Fang Liu, Kun Wu, Zhi Yong
Copyright Year	2012
Abstract	In this article, a simple but robust nanofluidic interface was introduced directly on a chip comprised of commercially available fused silica capillary with ready-made microchannel, and efficient on chip electrokinetic stacking of DNA was successfully demonstrated based on ion concentration polarization (ICP) effect. The nanofluidic interface was established by casting ion exchange polymer resin (Nafion) into a sub-microfracture (~650 nm) prepared on the capillary manually. The width of the fracture was electrically measured with the aide of a mathematic fracture model and confirmed by scanning electronic microscope. Obvious ICP effect was observed both by online microscopic fluorescent imaging and post laser induced fluorescence detection. SYBR Green I labeled dsDNA was stacked at the nanofluidic interface inside the microchannel (cathode side) with a concentration factor of 103 within 15 s. As high as 800 V was applied through the interface without any damage. The main materials are all commercially available, and no advanced microfabrication facilities are involved in the preparation of the chip.
Starting Page	69
Ending Page	76
Page Count	8
File Format	PDF
ISSN	16134982
Journal	Microfluidics and Nanofluidics
Volume Number	14
Issue Number	1-2
e-ISSN	16134990
Language	English
Publisher	Springer-Verlag
Publisher Date	2012-07-05
Publisher Place	Berlin, Heidelberg
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Nanofluidic interface Fused silica capillary Nafion Microfracture Ion concentration polarization DNA Electrokinetic stacking Engineering Fluid Dynamics Biomedical Engineering Analytical Chemistry Nanotechnology and Microengineering
Content Type	Text
Resource Type	Article
Subject	Nanoscience and Nanotechnology Materials Chemistry Condensed Matter Physics Electronic, Optical and Magnetic Materials

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