NDLI: Disk-like hydrogel bead-based immunofluorescence staining toward identification and observation of circulating tumor cells

Content Provider	Springer Nature Link
Author	Cai, Bo Guo, Feng Zhao, Libo He, Rongxiang Chen, Boran He, Zhaobo Yu, Xiaolei Guo, Shishang Xiong, Bin Liu, Wei Zhao, Xingzhong
Copyright Year	2013
Abstract	Assays toward analysis of rare heterogeneous cells among identical specimen raise a significant challenge in many cell biological studies and clinical diagnosis applications. In this work, we report a disk-like hydrogel bead-based stratagem for rare cell researches at single cell level after a facile microfluidic-based particle synthesis approach. Cells of interested can be encapsulated into alginate droplets which are subsequently solidified into disk-like calcium alginate hydrogel beads and the bead size and cell number inside can be precisely controlled. Due to stability, permeability and disk-like shape of calcium alginate beads, cells immobilized in the disk-like beads can be treated with different chemicals with limited mechanical or fluidic operation influences and observed without distortion comparing with conventional methods or droplet microfluidic methods. Identification of circulating tumor cells, related to early-stage cancer diagnosis, is targeted to demonstrate the potential of our technique in rare cell analysis. This hydrogel bead-based stratagem is performed in immunofluorescence staining treatment and observation of cancer cells from normal hematological cells in blood sample. This method would have a great potential in single cell immobilization, manipulations and observation for biochemical cellular assays of rare cells.
Starting Page	29
Ending Page	37
Page Count	9
File Format	PDF
ISSN	16134982
Journal	Microfluidics and Nanofluidics
Volume Number	16
Issue Number	1-2
e-ISSN	16134990
Language	English
Publisher	Springer Berlin Heidelberg
Publisher Date	2013-05-19
Publisher Place	Berlin, Heidelberg
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Microfluidics Disk-like hydrogel beads Cell encapsulation Immunofluorescence staining Engineering Fluid Dynamics Biomedical Engineering Analytical Chemistry Nanotechnology and Microengineering
Content Type	Text
Resource Type	Article
Subject	Nanoscience and Nanotechnology Materials Chemistry Condensed Matter Physics Electronic, Optical and Magnetic Materials

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