NDLI: Detection of Tobamo-Viruses in sycamore maple (Acer pseudoplatanus L.) exhibiting mottling and leaf deformation

Content Provider	Springer Nature Link
Author	Führling, Martina Büttner, Carmen
Copyright Year	1998
Abstract	Bergahorn (Acer pseudoplatanus L.) mit virusverdächtigen Symptomen wie einer Scheckung, zum Teil mit Andeutung eines Mosaiks, und Blattdeformationen wurde auf eine Infektion mit Tobamo-Viren hin überprüft. Während mit der ELISA-Testung kein Erregernachweis geführt werden konnte, waren Nukleinsäurefragmente von Tobamoviren mit Hilfe der Dot-blot Hybridisierung darzustellen. Adsorptionspräparate von erkranktem Blattmaterial ließen in der elektronenmikroskopischen Untersuchung starre Stäbchen mit einer Länge von ungefähr 0,3 µm erkennen, die der Tobamovirusgruppe zuzuordnen sind. Bisher konnten aus Bergahorn keine Viren nachgewiesen werden. Die Erfüllung der Koch’schen Postulate als Nachweis einer infektiösen Erkrankung wird derzeitig angestrebt.Sycamore maple (Acer pseudoplatanus L.) exhibiting virus-like leaf symptoms such as leaf deformation and mottling, partly implicating a mosaic, was tested by different methods to verify an infection with viruses belonging to the tobamovirus group. Whereas the ELISA-testing did not lead to the detection of the pathogen specific nucleic acid fragments could be shown by dot-blot hybridization. The finding was verified through the electronmicroscopic visualization of rodshaped particles with a length of approximately 0,3 µm. These particles could be related to the tobamovirus group. So far there is no report concerning the detection of viruses from sycamore maple. To fulfill Koch’s postulates investigations confirming the observed symptoms as an infectious disease are under way.
Starting Page	92
Ending Page	97
Page Count	6
File Format	PDF
ISSN	00158003
Journal	Forstwissenschaftliches Centralblatt vereinigt mit Tharandter forstliches Jahrbuch
Volume Number	117
Issue Number	1-6
e-ISSN	14390337
Language	English
Publisher	Springer Berlin Heidelberg
Publisher Date	1998-12-01
Publisher Place	Berlin, Heidelberg
Access Restriction	Subscribed
Subject Keyword	Forestry Plant Sciences Plant Ecology
Content Type	Text
Resource Type	Article
Subject	Plant Science Forestry

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