NDLI: Contribution of Steric Repulsion to Retention on an Octadecylsiloxane-Bonded Silica Stationary Phase in Reversed-Phase Liquid Chromatography

Content Provider	Springer Nature Link
Author	Poole, C. F. Ahmed, H. Kiridena, W. DeKay, C. Koziol, W. W.
Copyright Year	2005
Abstract	Plots of the retention factor against mobile phase composition were used to organize a varied group of solutes into three categories according to their retention mechanism on an octadecylsilioxane-bonded silica stationary phase, AscentisTM C18, with acetonitrile-water and methanol-water mobile phase compositions containing 10–70% (v/v) organic solvent. The solutes in category 1 could be fit to a general retention model, Eq. (1), and exhibited normal retention behavior for the full composition range. The solutes in category 2 exhibited normal retention behavior at high organic solvent compositions with a discontinuity at low organic solvent mobile phase compositions. The solutes in category 3 exhibited a pronounced step or plateau in the middle region of the retention plots with a retention mechanism similar to category 1 at mobile phase compositions after the discontinuity and a different retention mechanism before the discontinuity. Selecting solutes and appropriate composition ranges from the three categories where a single retention mechanism was operative allowed modeling of the experimental retention factors using the solvation parameter model. These models were then used to predict retention factors for solutes excluded from the models. The overwhelming number of residual values, here defined as the difference between experimental and model predicted retention factors for the excluded solutes, could be explained by contributions from steric repulsion. The latter defined as the inability of solutes to fully insert themselves into the solvated stationary phase because of their size or conformation. Steric repulsion resulted in a systematic reduction in retention compared with predicted values for the fully inserted solute. The bonding density of the stationary phase; the type and composition of the mobile phase; and the size, conformation, type and number of functional groups on the solute are shown to affect the contribution of steric repulsion to the retention mechanism.
Starting Page	553
Ending Page	561
Page Count	9
File Format	PDF
ISSN	00095893
Journal	Chromatographia
Volume Number	62
Issue Number	11-12
e-ISSN	16121112
Language	English
Publisher	Vieweg Verlag
Publisher Date	2005-12-05
Publisher Place	Wiesbaden
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Column liquid chromatography Solvation parameter model Ascentis C18 Steric repulsion Analytical Chemistry Organic Chemistry Biochemistry Plant Sciences Pharmacy Measurement Science, Instrumentation
Content Type	Text
Resource Type	Article
Subject	Organic Chemistry Analytical Chemistry Biochemistry Clinical Biochemistry

Sl.	Authority	Responsibilities	Communication Details
1	Ministry of Education (GoI), Department of Higher Education	Sanctioning Authority	https://www.education.gov.in/ict-initiatives
2	Indian Institute of Technology Kharagpur	Host Institute of the Project: The host institute of the project is responsible for providing infrastructure support and hosting the project	https://www.iitkgp.ac.in
3	National Digital Library of India Office, Indian Institute of Technology Kharagpur	The administrative and infrastructural headquarters of the project	Dr. B. Sutradhar bsutra@ndl.gov.in
4	Project PI / Joint PI	Principal Investigator and Joint Principal Investigators of the project	Dr. B. Sutradhar bsutra@ndl.gov.in Prof. Saswat Chakrabarti will be added soon
5	Website/Portal (Helpdesk)	Queries regarding NDLI and its services	support@ndl.gov.in
6	Contents and Copyright Issues	Queries related to content curation and copyright issues	content@ndl.gov.in
7	National Digital Library of India Club (NDLI Club)	Queries related to NDLI Club formation, support, user awareness program, seminar/symposium, collaboration, social media, promotion, and outreach	clubsupport@ndl.gov.in
8	Digital Preservation Centre (DPC)	Assistance with digitizing and archiving copyright-free printed books	dpc@ndl.gov.in
9	IDR Setup or Support	Queries related to establishment and support of Institutional Digital Repository (IDR) and IDR workshops	idr@ndl.gov.in

System Maps for RP-LC on an Octadecylsiloxane-Bonded Silica Stationary Phase (SunFire C18)

Insights into the Retention Mechanisms on Perfluorohexylpropylsiloxane-Bonded (Fluophase-RP) and Octadecylsiloxane-Bonded (Betasil C18) Stationary Phases Based on the Same Silica Substrate in RP-LC

Reversed-phase chromatography on a polar endcapped octadecylsiloxane-bonded stationary phase with water as the mobile phase

Insights into the Retention Mechanism on a Pentafluorophenylpropylsiloxane-Bonded Silica Stationary Phase (Discovery HS F5) in RP-LC

Evaluation of the retention characteristics of ferrocene-bonded silica stationary phase and other silica-based reversed phases for HPLC using linear solvation energy relationships

A novel method for the preparation of C18 ester-bonded RP-HPLC packings

Synthesis and evaluation of a ferrocene-bonded silica phase for reversed-phase high-performance liquid chromatography

System Maps for XTerra MS C18: Effect of Solvent Type on Selectivity in Reversed-Phase Liquid Chromatography

Comparison of the Separation Characteristics of the Organic–Inorganic Hybrid Octadecyl Stationary Phases XTerra MS C18 and XBridge C18 and Shield RP18 in RPLC

Contribution of Steric Repulsion to Retention on an Octadecylsiloxane-Bonded Silica Stationary Phase in Reversed-Phase Liquid Chromatography

Similar Documents

System Maps for RP-LC on an Octadecylsiloxane-Bonded Silica Stationary Phase (SunFire C18)

Insights into the Retention Mechanisms on Perfluorohexylpropylsiloxane-Bonded (Fluophase-RP) and Octadecylsiloxane-Bonded (Betasil C18) Stationary Phases Based on the Same Silica Substrate in RP-LC

Reversed-phase chromatography on a polar endcapped octadecylsiloxane-bonded stationary phase with water as the mobile phase

Insights into the Retention Mechanism on a Pentafluorophenylpropylsiloxane-Bonded Silica Stationary Phase (Discovery HS F5) in RP-LC

Evaluation of the retention characteristics of ferrocene-bonded silica stationary phase and other silica-based reversed phases for HPLC using linear solvation energy relationships

A novel method for the preparation of C18 ester-bonded RP-HPLC packings

Synthesis and evaluation of a ferrocene-bonded silica phase for reversed-phase high-performance liquid chromatography

System Maps for XTerra MS C18: Effect of Solvent Type on Selectivity in Reversed-Phase Liquid Chromatography

Comparison of the Separation Characteristics of the Organic–Inorganic Hybrid Octadecyl Stationary Phases XTerra MS C18 and XBridge C18 and Shield RP18 in RPLC

Contribution of Steric Repulsion to Retention on an Octadecylsiloxane-Bonded Silica Stationary Phase in Reversed-Phase Liquid Chromatography