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| Content Provider | Springer Nature Link |
|---|---|
| Author | Kiridena, W. Poole, C. F. Koziol, W. W. |
| Copyright Year | 2003 |
| Abstract | SynergiTM Hydro-RP is a polar endcapped octadecylsiloxane-bonded silica packing for reversed-phase chromatography compatible with water as a mobile phase. The effect of temperature on retention and selectivity for a varied group of compounds is evaluated from plots of the retention factors against temperature and by application of the solvation parameter model. Retention is shown to increase with the size and capability of the solute for electron lone pair interactions and is reduced by hydrogen-bonding and dipole-type interactions. Increasing temperature over the range 25 to 65°C reduces retention with only a small affect on selectivity. Increasing temperature effectively increases the phase ratio for the column and diminishes the relative contribution of electron lone pair interactions with the stationary phase. The SynergiTM Hydro-RP column in suitable for the separation of small polar molecules with good peak shape and reasonable efficiency in an acceptable separation time. |
| Starting Page | 703 |
| Ending Page | 707 |
| Page Count | 5 |
| File Format | |
| ISSN | 00095893 |
| Journal | Chromatographia |
| Volume Number | 57 |
| Issue Number | 11-12 |
| e-ISSN | 16121112 |
| Language | English |
| Publisher | Vieweg Verlag |
| Publisher Date | 2003-01-01 |
| Publisher Place | Wiesbaden |
| Access Restriction | One Nation One Subscription (ONOS) |
| Subject Keyword | Column liquid chromatography Solvation parameter model Retention mechanism Temperature effect on retention Water as mobile phase Analytical Chemistry Organic Chemistry Pharmacy Biochemistry Plant Sciences Measurement Science, Instrumentation |
| Content Type | Text |
| Resource Type | Article |
| Subject | Organic Chemistry Analytical Chemistry Biochemistry Clinical Biochemistry |
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