NDLI: Abnormal phenomenon of the dependence of the retention factors for uncharged species on applied voltage in capillary electrochromatography

Content Provider	Springer Nature Link
Author	Ping, Guichen Zhang, Weibing Zhang, Lin Zhang, Lihua Schmitt Kopplin, Ph. Kettrup, A. Zhang, Yukui
Copyright Year	2003
Abstract	Polymethacrylate-based monolithic columns were prepared for capillary electrochromatography (CEC) byin situ copolymerization of butyl methacrylate (BMA), 2-acrylamido-2-methyl-1-propanesulfonic acid (AMPS), and ethylene dimethacrylate (EDMA) in the presence of a porogen in fused-silica capillaries of 100 μm I.D. The abnormal phenomenon that retention factors for neutral species decreases with applied voltage in CEC was observed. Capillary electrophoresis (CE) instruments usually require a period of time to increase voltage from 0 kV to desired value, which is called as ramp time. Such ramp time and any error in the determination of dead time should be taken into account during the accurate calculation of retention factors. After the correction of the retention factors, the plots of the corrected factors for alkylbenzene versus applied voltage were made, the absolute value of the plot slopes are less than 1.8×10−4, which indicates that the corrected retention times for neutral species do not show any dependence on applied voltage. Further, the plats of the corrected retention times for acidic and basic compounds versus the reciprocal of applied voltage were drawn, where the target compounds were eluted in neutral form. The very nice linearity of the plots was obtained. The linear correlation coefficients are over 0.999. Here, the slopes of the plots represent $$\frac{{L_{eff} \cdot L_{tot} \cdot \left( {l + k} \right)}}{{\mu eo}}$$ , which indicates that retention factors for the test compounds are constants under a given separation condition.
Starting Page	777
Ending Page	781
Page Count	5
File Format	PDF
ISSN	00095893
Journal	Chromatographia
Volume Number	57
Issue Number	11-12
e-ISSN	16121112
Language	English
Publisher	Vieweg Verlag
Publisher Date	2003-01-01
Publisher Place	Wiesbaden
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Capillary electrochromatography Polymer monolithic column Applied valtage Retention factor Analytical Chemistry Organic Chemistry Pharmacy Biochemistry Plant Sciences Measurement Science, Instrumentation
Content Type	Text
Resource Type	Article
Subject	Organic Chemistry Analytical Chemistry Biochemistry Clinical Biochemistry

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