NDLI: An Advanced LC–MS/MS Platform for the Analysis of Specialized Pro-Resolving Lipid Mediators

Content Provider	Springer Nature Link
Author	Jónasdóttir, Hulda S. Ioan Facsinay, Andreea Kwekkeboom, Joanneke Brouwers, Hilde Zuurmond, Anne Marie Toes, René Deelder, André M. Giera, Martin
Copyright Year	2014
Abstract	Here we present an advanced platform for the analysis of specialized pro-resolving mediators (SPM), including a set of pro-inflammatory lipids and respective biochemical pathway markers. The platform is characterized by a largely simplified sample preparation protocol, employing methanol protein-precipitation instead of solid phase extraction. Sample preparation and analysis can be carried out in 96-well format, ensuring higher throughput. In addition, faster analysis times and higher sensitivities have been achieved when compared to other platforms. In total 36 lipid mediators—SPM, their pathway markers and a set of pro-inflammatory lipids including three internal standards—are analyzed within an 11 min LC–MS/MS run. The method was validated using human plasma, including repeatability, linearity, recovery, matrix effects, and accuracy. The presented platform was applied in the analysis of synovial fluid post-mortem samples and cellular extracts from polymorphonuclear cells as well as whole blood treated with ionophore in the presence of the polyunsaturated fatty acid eicosapentaenoic acid.
Starting Page	391
Ending Page	401
Page Count	11
File Format	PDF
ISSN	00095893
Journal	Chromatographia
Volume Number	78
Issue Number	5-6
e-ISSN	16121112
Language	English
Publisher	Springer Berlin Heidelberg
Publisher Date	2014-10-17
Publisher Place	Berlin, Heidelberg
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	LC–MS Inflammation resolution Pro-resolving mediators Synovial fluid LTE4 EPA Chromatography Proteomics Pharmacy Laboratory Medicine
Content Type	Text
Resource Type	Article
Subject	Organic Chemistry Analytical Chemistry Biochemistry Clinical Biochemistry

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