NDLI: LC–MS–MS Method for the Simultaneous Determination of Donepezil Enantiomers in Plasma

Content Provider	Springer Nature Link
Author	Iordachescu, Adriana Silvestro, Luigi Tudoroniu, Ariana Rizea Savu, Simona Ciuca, Valentin
Copyright Year	2012
Abstract	This paper presents a new analytical method with adequate sensitivity, precision, accuracy, and specificity to quantitatively determine (−)-donepezil and (+)-donepezil in plasma, at concentrations that can be expected in real samples of pharmacokinetic studies. This method combines a HPLC separation on cellulose tris (3,5-dimethylphenyl carbamate) coated on 5-μm silica-gel column known as CHIRALCEL OD–RH eluted with mobile phase consisting of acetonitrile and ammonium bicarbonate and MS–MS detection. Under the HPLC conditions used (−)-donepezil and (+)-donepezil, as well as the enantiomers of the internal standard d7-donepezil, eluted at 5 and 6.25 min, respectively. The curve fittings were optimal for the entire calibration range (0.05–25.0 ng mL−1) with correlation coefficients (r) = 0.999 for (−)-donepezil and (r) = 0.999 for (+)-donepezil (linear regression model with 1/x 2 weighing). The assay method showed a good specificity for donepezil enantiomers, and it could be successfully applied to pharmacokinetic studies.
Starting Page	857
Ending Page	866
Page Count	10
File Format	PDF
ISSN	00095893
Journal	Chromatographia
Volume Number	75
Issue Number	15-16
e-ISSN	16121112
Language	English
Publisher	Springer-Verlag
Publisher Date	2012-07-26
Publisher Place	Berlin, Heidelberg
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	LC–MS–MS HPLC Donepezil Enantiomers Pharmacokinetic Chiral stationary phase Chromatography Pharmacy Proteomics Laboratory Medicine
Content Type	Text
Resource Type	Article
Subject	Organic Chemistry Analytical Chemistry Biochemistry Clinical Biochemistry

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