NDLI: Effect of Suboptimal Sampling and Handling Conditions on Urinary Metabolic Profiles

Content Provider	Springer Nature Link
Author	Morello, Judit Nevedomskaya, Ekaterina Pacchiarotta, Tiziana Schoemaker, Bart Derks, Rico Voet, Nicoline B. M. Meissner, Axel Deelder, André M. Engelen, Baziel G. M. Mayboroda, Oleg A.
Copyright Year	2014
Abstract	Collection and storage of the clinical samples are crucial factors in the metabolomic workflows. However, with the expansion of metabolomics into the clinical domain and towards the large field studies in particular, the high sampling/storage standards practiced in the tightly controlled hospital environment cannot always be guaranteed. Thus, if the samples are exposed to suboptimal conditions and their integrity is compromised should they be discarded? Or such samples retain physiologically relevant information and can be of use? To explore the options we analyzed 117 urine samples that were collected under two different conditions. Part of the samples were collected within a clinical setting under optimal conditions, another part by patients at home and shipped to the hospital by mail. All samples were analyzed by liquid chromatography–mass spectrometry (LC–MS) and proton nuclear magnetic resonance (1H NMR) spectroscopy. Multivariate modelling revealed clear differences between the two sampling conditions for both LC–MS and 1H NMR data sets. However, the differential metabolites appeared to be platform-specific, which clearly emphasizes the complementary nature of both techniques. The analysis of the samples that were exposed to suboptimal conditions revealed that age and body mass index remain as dominant traits of the metabolic profile, although their influence was stronger for LC–MS data. In conclusion, although it is important to ensure adequate sample collection and storage conditions, urine samples that do not fulfil these criteria still retain valuable physiological information and as such thus they could be of use for metabolomic studies when no alternative is available.
Starting Page	429
Ending Page	434
Page Count	6
File Format	PDF
ISSN	00095893
Journal	Chromatographia
Volume Number	78
Issue Number	5-6
e-ISSN	16121112
Language	English
Publisher	Springer Berlin Heidelberg
Publisher Date	2014-10-19
Publisher Place	Berlin, Heidelberg
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Metabolomics Urine Bacterial metabolism Principal component analysis Mass spectrometry Chromatography Proteomics Pharmacy Laboratory Medicine
Content Type	Text
Resource Type	Article
Subject	Organic Chemistry Analytical Chemistry Biochemistry Clinical Biochemistry

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1	Ministry of Education (GoI), Department of Higher Education	Sanctioning Authority	https://www.education.gov.in/ict-initiatives
2	Indian Institute of Technology Kharagpur	Host Institute of the Project: The host institute of the project is responsible for providing infrastructure support and hosting the project	https://www.iitkgp.ac.in
3	National Digital Library of India Office, Indian Institute of Technology Kharagpur	The administrative and infrastructural headquarters of the project	Dr. B. Sutradhar bsutra@ndl.gov.in
4	Project PI / Joint PI	Principal Investigator and Joint Principal Investigators of the project	Dr. B. Sutradhar bsutra@ndl.gov.in Prof. Saswat Chakrabarti will be added soon
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