NDLI: Tetrazole-Functionalized Silica for Hydrophilic Interaction Chromatography of Polar Solutes

Content Provider	Springer Nature Link
Author	Dai, Xiaojun Qian, Xiaolei Gong, Bolin Wei, Yinmao
Copyright Year	2011
Abstract	In this work, tetrazole-functionalized stationary phase was prepared with nitrile-modified silica by an ammonium-catalyzed (3 + 2) azide-nitrile cycloaddition reaction. The prepared stationary phase was used for separation of nucleobases and nucleosides by hydrophilic interaction chromatography (HILIC) mode. A typical HILIC mechanism was observed at higher content of acetonitrile (>85%, v/v) in the mobile phase. The retention mechanism of the column was investigated by the models used for describing partitioning and surface adsorption through adjustment ratio of water in the mobile phase, and by the influence of salt concentration, buffer pH, and temperature on the retention of solutes. The results illustrated that the surface adsorption through hydrogen bonding dominated the retention behavior of nucleobases/nucleosides under HILIC mode. From the separation ability, the tetrazole-functionalized stationary phase could become a valuable alternative for the separation of the compounds concerned.
Starting Page	865
Ending Page	870
Page Count	6
File Format	PDF
ISSN	00095893
Journal	Chromatographia
Volume Number	73
Issue Number	9-10
e-ISSN	16121112
Language	English
Publisher	Springer-Verlag
Publisher Date	2011-02-26
Publisher Place	Berlin, Heidelberg
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Hydrophilic interaction chromatography Tetrazole-functionalized silica Nucleobases Nucleosides Retention mechanism Plant Sciences Pharmacy Organic Chemistry Proteomics Biochemistry Analytical Chemistry
Content Type	Text
Resource Type	Article
Subject	Organic Chemistry Analytical Chemistry Biochemistry Clinical Biochemistry

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