NDLI: GC–MS Simultaneous Determination of Enalapril and Enalaprilat in Human Plasma: Application to a Clinical Pharmacokinetic Study

Content Provider	Springer Nature Link
Author	Spanakis, Marios Niopas, Ioannis
Copyright Year	2010
Abstract	Enalapril is a prodrug that is metabolized to its main active metabolite, enalaprilat that is a potent inhibitor of angiotensin converting enzyme (ACE). A simple, rapid, sensitive, precise and accurate SIM GC–MS method, using solid-phase extraction and derivatization with methyl iodide, for the quantitative determination of enalapril and enalaprilat in human plasma was developed and validated. The ions at m/z 220 for enalapril, m/z 234 for enalaprilat and m/z 172 for internal standard, perindopril, were monitored. The calibration graphs for the analytes were linear in the 5–160 ng mL−1 concentration range (r > 0.999). Intra-day and inter-day precision for enalapril ranged from 2.4 to 3.5 and 3.9 to 7.9%, and for enalaprilat from 5.3 to 7.8 and 6.7 to 9.1%. Accuracy was better than 105.5% for both enalapril and enalaprilat. The method was sensitive with a lower limit of quantitation (LLOQ) of 2 ng mL−1 for both analytes and was not interfered by other plasma components. The method was applied for the determination of enalaprilat in a pharmacokinetic study after single oral administration of 20 mg enalapril to 24 healthy subjects.
Starting Page	957
Ending Page	962
Page Count	6
File Format	PDF
ISSN	00095893
Journal	Chromatographia
Volume Number	72
Issue Number	9-10
e-ISSN	16121112
Language	English
Publisher	Vieweg Verlag
Publisher Date	2010-08-31
Publisher Place	Wiesbaden
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Gas chromatography-mass spectrometry Solid phase extraction Pharmacokinetic study Enalapril and enalaprilat Measurement Science and Instrumentation Plant Sciences Biochemistry Pharmacy Organic Chemistry Analytical Chemistry
Content Type	Text
Resource Type	Article
Subject	Organic Chemistry Analytical Chemistry Biochemistry Clinical Biochemistry

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