NDLI: Ethanol production from sorghum by a dilute ammonia pretreatment

Content Provider	Springer Nature Link
Author	Salvi, D. A. Aita, G. M. Robert, D. Bazan, V.
Copyright Year	2009
Abstract	Sorghum fibers were pretreated with ammonium hydroxide and the effectiveness of the pretreatment evaluated by enzyme hydrolysis and ethanol production. The treatment was carried out by mixing sorghum fibers, ammonia, and water at a ratio of 1:0.14:8 at 160°C for 1 h under 140–160 psi pressure. Approximately 44% lignin and 35% hemicellulose were removed during the process. Untreated and dilute-ammonia-treated fibers at 10% dry solids were hydrolyzed using combinations of commercially available enzymes, Spezyme CP and Novozyme 188. Enzyme combinations were tested at full strength (60 FPU Spezyme CP and 64 CBU Novozyme 188/g glucan) and at half strength (30 FPU Spezyme CP and 32 CBU Novozyme 188/g glucan). Biomass enzyme hydrolysis was conducted for 24 h. Saccharomyces cerevisiae D5A was added post hydrolysis for conversion of glucose to ethanol. Theoretical cellulose yields for treated biomass were 84% and 73%, and hemicellulose yields were 73% and 55% for full strength and half strength, respectively. Average cellulose yield was 38% and hemicellulose yield was 14.5% for untreated biomass. Ethanol yields were 25 g/100 g dry biomass and 21 g/100 g dry biomass for full strength and half strength enzyme concentrations, respectively. Controls averaged 10 g ethanol/100 g dry biomass.
Starting Page	27
Ending Page	34
Page Count	8
File Format	PDF
ISSN	13675435
Journal	Journal of Industrial Microbiology and Biotechnology
Volume Number	37
Issue Number	1
e-ISSN	14765535
Language	English
Publisher	Springer-Verlag
Publisher Date	2009-10-01
Publisher Place	Berlin, Heidelberg
Access Restriction	Subscribed
Subject Keyword	Ammonia treatment Sorghum Ethanol Lignocellulosic Biomass Hydrolysis Microbiology Bioinformatics Biotechnology Genetic Engineering Inorganic Chemistry Biochemistry
Content Type	Text
Resource Type	Article
Subject	Medicine Bioengineering Applied Microbiology and Biotechnology Biotechnology

Sl.	Authority	Responsibilities	Communication Details
1	Ministry of Education (GoI), Department of Higher Education	Sanctioning Authority	https://www.education.gov.in/ict-initiatives
2	Indian Institute of Technology Kharagpur	Host Institute of the Project: The host institute of the project is responsible for providing infrastructure support and hosting the project	https://www.iitkgp.ac.in
3	National Digital Library of India Office, Indian Institute of Technology Kharagpur	The administrative and infrastructural headquarters of the project	Dr. B. Sutradhar bsutra@ndl.gov.in
4	Project PI / Joint PI	Principal Investigator and Joint Principal Investigators of the project	Dr. B. Sutradhar bsutra@ndl.gov.in Prof. Saswat Chakrabarti will be added soon
5	Website/Portal (Helpdesk)	Queries regarding NDLI and its services	support@ndl.gov.in
6	Contents and Copyright Issues	Queries related to content curation and copyright issues	content@ndl.gov.in
7	National Digital Library of India Club (NDLI Club)	Queries related to NDLI Club formation, support, user awareness program, seminar/symposium, collaboration, social media, promotion, and outreach	clubsupport@ndl.gov.in
8	Digital Preservation Centre (DPC)	Assistance with digitizing and archiving copyright-free printed books	dpc@ndl.gov.in
9	IDR Setup or Support	Queries related to establishment and support of Institutional Digital Repository (IDR) and IDR workshops	idr@ndl.gov.in

Dilute Ammonia Pretreatment of Sorghum and Its Effectiveness on Enzyme Hydrolysis and Ethanol Fermentation

Mushroom spent straw: a potential substrate for an ethanol-based biorefinery

Simulation of integrated first and second generation bioethanol production from sugarcane: comparison between different biomass pretreatment methods

Dilute ammonia pretreatment of sorghum and its effectiveness on enzyme hydrolysis and ethanol fermentation.

Evaluation of hardboard manufacturing process wastewater as a feedstream for ethanol production

Reducing acid in dilute acid pretreatment and the impact on enzymatic saccharification

Hot-water pretreatment of cattails for extraction of cellulose

A study on the pretreatment of a sugarcane bagasse sample with dilute sulfuric acid

Increased ethanol production by deletion of HAP4 in recombinant xylose-assimilating Saccharomyces cerevisiae

Ethanol production from sorghum by a dilute ammonia pretreatment

Similar Documents

Dilute Ammonia Pretreatment of Sorghum and Its Effectiveness on Enzyme Hydrolysis and Ethanol Fermentation

Mushroom spent straw: a potential substrate for an ethanol-based biorefinery

Simulation of integrated first and second generation bioethanol production from sugarcane: comparison between different biomass pretreatment methods

Dilute ammonia pretreatment of sorghum and its effectiveness on enzyme hydrolysis and ethanol fermentation.

Evaluation of hardboard manufacturing process wastewater as a feedstream for ethanol production

Reducing acid in dilute acid pretreatment and the impact on enzymatic saccharification

Hot-water pretreatment of cattails for extraction of cellulose

A study on the pretreatment of a sugarcane bagasse sample with dilute sulfuric acid

Increased ethanol production by deletion of HAP4 in recombinant xylose-assimilating Saccharomyces cerevisiae

Ethanol production from sorghum by a dilute ammonia pretreatment