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| Content Provider | Springer Nature Link |
|---|---|
| Author | Matsushika, Akiri Hoshi, Tamotsu |
| Copyright Year | 2015 |
| Abstract | The Saccharomyces cerevisiae HAP4 gene encodes a transcription activator that plays a key role in controlling the expression of genes involved in mitochondrial respiration and reductive pathways. This work examines the effect of knockout of the HAP4 gene on aerobic ethanol production in a xylose-utilizing S. cerevisiae strain. A hap4-deleted recombinant yeast strain (B42-DHAP4) showed increased maximum concentration, production rate, and yield of ethanol compared with the reference strain MA-B42, irrespective of cultivation medium (glucose, xylose, or glucose/xylose mixtures). Notably, B42-DHAP4 was capable of producing ethanol from xylose as the sole carbon source under aerobic conditions, whereas no ethanol was produced by MA-B42. Moreover, the rate of ethanol production and ethanol yield (0.44 g/g) from the detoxified hydrolysate of wood chips was markedly improved in B42-DHAP4 compared to MA-B42. Thus, the results of this study support the view that deleting HAP4 in xylose-utilizing S. cerevisiae strains represents a useful strategy in ethanol production processes. |
| Starting Page | 1623 |
| Ending Page | 1631 |
| Page Count | 9 |
| File Format | |
| ISSN | 13675435 |
| Journal | Journal of Industrial Microbiology and Biotechnology |
| Volume Number | 42 |
| Issue Number | 12 |
| e-ISSN | 14765535 |
| Language | English |
| Publisher | Springer Berlin Heidelberg |
| Publisher Date | 2015-10-05 |
| Publisher Place | Berlin, Heidelberg |
| Access Restriction | Subscribed |
| Subject Keyword | Saccharomyces cerevisiae HAP4 gene Xylose Ethanol production Lignocellulosic hydrolysate Microbiology Biochemistry Inorganic Chemistry Genetic Engineering Biotechnology Bioinformatics |
| Content Type | Text |
| Resource Type | Article |
| Subject | Medicine Bioengineering Applied Microbiology and Biotechnology Biotechnology |
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