NDLI: Microbe repelling coated stainless steel analysed by field emission scanning electron microscopy and physicochemical methods

Content Provider	Springer Nature Link
Author	Raulio, Mari Järn, Mikael Ahola, Juhana Peltonen, Jouko Rosenholm, Jarl B. Tervakangas, Sanna Kolehmainen, Jukka Ruokolainen, Timo Narko, Pekka Salkija Salonen, Mirja
Copyright Year	2008
Abstract	Coating of stainless steel with diamond-like carbon or certain fluoropolymers reduced or almost eliminated adhesion and biofilm growth of Staphylococcus epidermidis, Deinococcus geothermalis, Meiothermus silvanus and Pseudoxanthomonas taiwanensis. These species are known to be pertinent biofilm formers on medical implants or in the wet-end of paper machines. Field emission scanning electron microscopic analysis showed that Staph. epidermidis, D. geothermalis and M. silvanus grew on stainless steel using thread-like organelles for adhesion and biofilm formation. The adhesion threads were fewer in number on fluoropolymer-coated steel than on plain steel and absent when the same strains were grown in liquid culture. Psx. taiwanensis adhered to the same surfaces by a mechanism involving cell ghosts on which the biofilm of live cells grew. Hydrophilic (diamond-like carbon) or hydrophobic (fluoropolymer) coatings reduced the adherence of the four test bacteria on different steels. Selected topographic parameters, including root-mean-square roughness (S q), skewness (S sk) and surface kurtosis (S ku), were analysed by atomic force microscopy. The surfaces that best repelled microbial adhesion of the tested bacteria had higher skewness values than those only slightly repelling. Water contact angle, measured (θ m) or roughness corrected (θ y), affected the tendency for biofilm growth in a different manner for the four test bacteria.
Starting Page	751
Ending Page	760
Page Count	10
File Format	PDF
ISSN	13675435
Journal	Journal of Industrial Microbiology and Biotechnology
Volume Number	35
Issue Number	7
e-ISSN	14765535
Language	English
Publisher	Springer-Verlag
Publisher Date	2008-04-01
Publisher Place	Berlin, Heidelberg
Access Restriction	Subscribed
Subject Keyword	Ultrastructure Bacterial adhesion Field emission scanning electron microscope (FESEM) Physicochemical parameters Contact angle Topographic analysis Microbial Genetics and Genomics Microbiology Bioinformatics Biochemistry Genetic Engineering Biotechnology
Content Type	Text
Resource Type	Article
Subject	Medicine Bioengineering Applied Microbiology and Biotechnology Biotechnology

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