NDLI: Activity of abrB310 promoter in wild type and spo0A-deficient strains of Clostridium acetobutylicum

Content Provider	Springer Nature Link
Author	Scotcher, Miles C. Bennett, George N.
Copyright Year	2008
Abstract	In Clostridium acetobutylicum, abrB310 is transcribed from two transcription start sites (designated A1 and A2) forming an abundant large, and a five- to tenfold less abundant small transcript, respectively throughout exponential, acidogenic growth and early in the transitional period to stationary, solventogenic growth. β-galactosidase reporter vectors were constructed to compare the transcriptional activity of the entire abrB310 promoter and the A1 and A2 transcription start sites individually. In stark contrast to the primer extension data, the A2 start site was threefold more active than the entire promoter, which was threefold more active than the A1 start site in wild type C. acetobutylicum. The activity expressed from all three reporter vectors declined as the cultures transitioned from exponential to stationary growth. In the spo0A-deficient strain SKO1, reporter vector activity continued for 10 h into stationary growth. The removal of the putative Spo0A binding site from all three vectors had no significant effect on promoter activity in either wild type or SKO1. We conclude that the presence of both the A1 and A2 transcription start sites is required for the correct control of abrB310 expression, and that AbrB310 is necessary but not sufficient for the correct transition between acidogenic and solventogenic growth.
Starting Page	743
Ending Page	750
Page Count	8
File Format	PDF
ISSN	13675435
Journal	Journal of Industrial Microbiology and Biotechnology
Volume Number	35
Issue Number	7
e-ISSN	14765535
Language	English
Publisher	Springer-Verlag
Publisher Date	2008-03-18
Publisher Place	Berlin, Heidelberg
Access Restriction	Subscribed
Subject Keyword	Clostridium Sporulation Solvent Metabolite Promoter Microbial Genetics and Genomics Microbiology Bioinformatics Biochemistry Genetic Engineering Biotechnology
Content Type	Text
Resource Type	Article
Subject	Medicine Bioengineering Applied Microbiology and Biotechnology Biotechnology

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