NDLI: Complete nucleotide sequence of pGS18, a 62.8-kb plasmid from Geobacillus stearothermophilus strain 18

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Complete nucleotide sequence of pGS18, a 62.8-kb plasmid from Geobacillus stearothermophilus strain 18

Content Provider	Springer Nature Link
Author	Stuknyte, Milda Guglielmetti, Simone Mora, Diego Kuisiene, meda Parini, Carlo Citavicius, Donaldas
Copyright Year	2008
Abstract	The complete nucleotide sequence (62.8 kb) of pGS18, the largest sequenced plasmid to date from the species Geobacillus stearothermophilus, was determined. Computational analysis of sequence data revealed 65 putative open reading frames (ORFs); 38 were carried on one strand and 27 were carried on the other. These ORFs comprised 84.1% of the pGS18 sequence. Twenty-five ORFs (38.4%) were assigned to putative functions; four ORFs (6.2%) were annotated as pseudogenes. The amino acid sequences obtained from 29 ORFs (44.6%) had the highest similarity to hypothetical proteins of the other microorganisms, and seven (10.8%) had no significant similarity to any genes present in the current open databases. Plasmid replication region, strongly resembling that of the theta-type replicon, and genes encoding three different plasmid maintenance systems were identified, and a putative discontinuous transfer region was localized. In addition, we also found several mobile genetic elements and genes, responsible for DNA repair, distributed along the whole sequence of pGS18. The alignment of pGS18 with two other large indigenous plasmids of the genus Geobacillus highlighted the presence of well-conserved segments and has provided a framework that can be exploited to formulate hypotheses concerning the molecular evolution of these three plasmids.
Starting Page	415
Ending Page	429
Page Count	15
File Format	PDF
ISSN	14310651
Journal	Extremophiles
Volume Number	12
Issue Number	3
e-ISSN	14334909
Language	English
Publisher	Springer Japan
Publisher Date	2008-02-28
Publisher Place	Japan
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Geobacillus stearothermophilus Plasmid Complete nucleotide sequence Comparison Ecology Biotechnology Microbiology
Content Type	Text
Resource Type	Article
Subject	Medicine Molecular Medicine Microbiology

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