NDLI: The third plasmid pVV8 from Thermus thermophilus HB8: isolation, characterization, and sequence determination

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The third plasmid pVV8 from Thermus thermophilus HB8: isolation, characterization, and sequence determination

Content Provider	Springer Nature Link
Author	Ohtani, Naoto Tomita, Masaru Itaya, Mitsuhiro
Copyright Year	2011
Abstract	The extremely thermophilic bacterium Thermus thermophilus is a model organism for structural biology and systems biology, and the so-called “Structural and Functional Whole-Cell Project for T. thermophilus HB8” is in progress. The released genomic sequence of the strain HB8 is composed of chromosome, pTT27 megaplasmid, and pTT8 plasmid. In this paper, however, a third plasmid was demonstrated and its sequence was determined. Although this plasmid pVV8 had been reported before, limited information and an unfortunate dropout in the substrain, whose genomic sequence was determined, would have prevented the plasmid from coming to public attention. The intrinsic circular plasmid, which was estimated to be six to ten copies in a cell, is 81151 bp and its G + C content is 68%. Among the identified 91 ORFs, a single gene has been experimentally analyzed before and is known as xylose isomerase. The phnCDEGHIJKLMX operon related to phosphonate metabolism, alkaline phosphatase, putative transcriptional regulators, several sets of toxin–antitoxin system, and transposase-like ORFs are also encoded on the pVV8 plasmid. Although association with cell aggregation was the one phenotypic characteristic of the plasmid that had been reported, it was never confirmed. Comparison of T. thermophilus HB8 strains suggests that the pVV8 is nonessential for growth.
Starting Page	237
Ending Page	244
Page Count	8
File Format	PDF
ISSN	14310651
Journal	Extremophiles
Volume Number	16
Issue Number	2
e-ISSN	14334909
Language	English
Publisher	Springer Japan
Publisher Date	2012-01-03
Publisher Place	Japan
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Thermus thermophilus HB8 Extreme thermophile Plasmid Complete sequence pVV8 Ecology Microbiology Biotechnology
Content Type	Text
Resource Type	Article
Subject	Medicine Molecular Medicine Microbiology

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Identification of a replication initiation protein of the pVV8 plasmid from Thermus thermophilus HB8

Purification and characterization of a NADH oxidase from the thermophile Thermus thermophilus HB8

The nucleotide sequence of 5S rRNA from an extreme thermophile, Thermus thermophilus HB8.

Purification and characterization of thermostable serine proteases encoded by the genes ttha0099 and ttha01320 from Thermus thermophilus HB8

Localized synthesis of the outer envelope from Thermus thermophilus

Molecular cloning of the isocitrate dehydrogenase gene of an extreme thermophile, Thermus thermophilus HB8.

Molecular Cloning of the Isocitrate Dehydrogenase Gene of an Extreme Thermophile, Thermus thermophilus HB8

Aminoacyl-tRNA formation in the extreme thermophile Thermus thermophilus

Isolation and characterization of a bacteriophage infectious to an extreme thermophile, Thermus thermophilus HB8.

The third plasmid pVV8 from Thermus thermophilus HB8: isolation, characterization, and sequence determination

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The third plasmid pVV8 from Thermus thermophilus HB8: isolation, characterization, and sequence determination