NDLI: A xylan-degrading strain of Sulfolobus solfataricus: isolation and characterization of the xylanase activity

Content Provider	Springer Nature Link
Author	Cannio, Raffaele Prizito, Natascia Rossi, Mosè Morana, Alessandra
Copyright Year	2004
Abstract	Two strains (Oα and X2) of the hyperthermophilic crenarchaeon Sulfolobus solfataricus strain MT4 were selected and isolated for their ability to grow on xylan. Oα and X2, grown on media containing oat spelt xylan and birchwood xylan as the sole nutrient source, respectively, produced the same thermostable xylanase that was demonstrated to be inducible in xylan cultures. In an oat spelt medium, S. solfataricus Oα underwent interesting morphological changes in the cell envelope, exhibiting mobile appendages not present in the typical coccal shape. The enzyme was prevalently membrane associated and showed a molecular mass of approximately 57.0 kDa. It was also highly thermostable, with a half-life of 47 min at 100°C, and exhibited an optimal temperature and pH of 90°C and 7.0, respectively. Xylo-oligosaccharides were the enzymatic products of xylan hydrolysis, and the smallest degradation product was xylobiose, thus indicating that the enzyme was an endoxylanase. The enzyme was able to bind weakly to crystalline cellulose (Avicel) and more strongly to insoluble xylan in a substrate amount-and temperature-dependent manner.
Starting Page	117
Ending Page	124
Page Count	8
File Format	PDF
ISSN	14310651
Journal	Extremophiles
Volume Number	8
Issue Number	2
e-ISSN	14334909
Language	English
Publisher	Springer-Verlag
Publisher Date	2004-01-10
Publisher Place	Tokyo
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Archaea Sulfolobus solfataricus Xylan Xylanase
Content Type	Text
Resource Type	Article
Subject	Medicine Molecular Medicine Microbiology

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