NDLI: Biochemical characterization of two Cdc6/ORC1-like proteins from the crenarchaeon Sulfolobus solfataricus

Please wait, while we are loading the content...

The thiol-disulfide oxidoreductase system in the cold-adapted bacterium Pseudoalteromonas haloplanktis TAC 125: discovery of a novel disulfide oxidoreductase enzyme

Cellulase activity of a haloalkaliphilic anaerobic bacterium, strain Z-7026

Biochemical characterization of two Cdc6/ORC1-like proteins from the crenarchaeon Sulfolobus solfataricus

Fatty acid and DNA analyses of Permian bacteria isolated from ancient salt crystals reveal differences with their modern relatives

Characterization and gene cloning of a cold-active cellulase from a deep-sea psychrotrophic bacterium Pseudoalteromonas sp. DY3

Acknowledgement

Extremophiles : Volume 9

Extremophiles : Volume 8

Extremophiles : Volume 7

Extremophiles : Volume 6

Extremophiles : Volume 5

Extremophiles : Volume 4

Extremophiles : Volume 3

Extremophiles : Volume 2

Extremophiles : Volume 1

Biochemical characterization of two Cdc6/ORC1-like proteins from the crenarchaeon Sulfolobus solfataricus

Content Provider	Springer Nature Link
Author	Felice, Mariarita Esposito, Luca Rossi, Mosè Pisani, Francesca M.
Copyright Year	2005
Abstract	The biological role of archaeal proteins, homologous to the eukaryal replication initiation factors of cell division control (Cdc6) and origin recognition complex (ORC1), has not yet been clearly established. The hyperthermophilic crenarchaeon Sulfolobus solfataricus (referred to as Sso) possesses three Cdc6/ORC1-like factors, which are named Sso Cdc6-1, Cdc6-2 and Cdc6-3. This study is a report on the biochemical characterization of Sso Cdc6-1 and Cdc6-3. It has been found that either Sso Cdc6-1 or Cdc6-3 behave as monomers in solutions by gel filtration analyses. Both factors are able to bind to various single-stranded and double-stranded DNA ligands, but Sso Cdc6-3 shows a higher DNA-binding affinity. It has also been observed that either Sso Cdc6-1 or Cdc6-3 inhibit the DNA unwinding activity of the S. solfataricus homo-hexameric mini-chromosome maintenance (MCM)-like DNA helicase (Sso MCM); although they strongly stimulate the interaction of the Sso MCM with bubble-containing synthetic oligonucleotides. The study has also showed, with surface plasmon resonance measurements, that Sso Cdc6-2 physically interacts with either Sso Cdc6-1 or Sso Cdc6-3. These findings may provide important clues needed to understand the biological role that is played by each of these three Cdc6 factors during the DNA replication initiation process in the S. solfataricus cells.
Starting Page	61
Ending Page	70
Page Count	10
File Format	PDF
ISSN	14310651
Journal	Extremophiles
Volume Number	10
Issue Number	1
e-ISSN	14334909
Language	English
Publisher	Springer-Verlag
Publisher Date	2005-09-23
Publisher Place	Tokyo
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	DNA replication Cell division control protein 6 Origin recognition complex Archaea Sulfolobus solfataricus
Content Type	Text
Resource Type	Article
Subject	Medicine Molecular Medicine Microbiology

Sl.	Authority	Responsibilities	Communication Details
1	Ministry of Education (GoI), Department of Higher Education	Sanctioning Authority	https://www.education.gov.in/ict-initiatives
2	Indian Institute of Technology Kharagpur	Host Institute of the Project: The host institute of the project is responsible for providing infrastructure support and hosting the project	https://www.iitkgp.ac.in
3	National Digital Library of India Office, Indian Institute of Technology Kharagpur	The administrative and infrastructural headquarters of the project	Dr. B. Sutradhar bsutra@ndl.gov.in
4	Project PI / Joint PI	Principal Investigator and Joint Principal Investigators of the project	Dr. B. Sutradhar bsutra@ndl.gov.in Prof. Saswat Chakrabarti will be added soon
5	Website/Portal (Helpdesk)	Queries regarding NDLI and its services	support@ndl.gov.in
6	Contents and Copyright Issues	Queries related to content curation and copyright issues	content@ndl.gov.in
7	National Digital Library of India Club (NDLI Club)	Queries related to NDLI Club formation, support, user awareness program, seminar/symposium, collaboration, social media, promotion, and outreach	clubsupport@ndl.gov.in
8	Digital Preservation Centre (DPC)	Assistance with digitizing and archiving copyright-free printed books	dpc@ndl.gov.in
9	IDR Setup or Support	Queries related to establishment and support of Institutional Digital Repository (IDR) and IDR workshops	idr@ndl.gov.in

Biochemical Characterization of a CDC6-like Protein from the Crenarchaeon Sulfolobus solfataricus

Diversity of DNA Replication in the Archaea

Molecular determinants of origin discrimination by orc1 initiators in archaea (2010).

Identification and autonomous replication capability of a chromosomal replication origin from the archaeon Sulfolobus solfataricus

A CDC6-like Factor from the Archaea Sulfolobus solfataricus Promotes Binding of the Mini-chromosome Maintenance Complex to DNA

Biochemical evidence of a physical interaction between Sulfolobus solfataricus B-family and Y-family DNA polymerases

Modular organization of a Cdc6-like protein from the crenarchaeon Sulfolobus solfataricus

A novel thermostable protein-tag: optimization of the Sulfolobus solfataricus DNA- alkyl-transferase by protein engineering

The Cdc45·Mcm2–7·GINS Protein Complex in Trypanosomes Regulates DNA Replication and Interacts with Two Orc1-like Proteins in the Origin Recognition Complex

Biochemical characterization of two Cdc6/ORC1-like proteins from the crenarchaeon Sulfolobus solfataricus

Similar Documents

Biochemical Characterization of a CDC6-like Protein from the Crenarchaeon Sulfolobus solfataricus

Diversity of DNA Replication in the Archaea

Molecular determinants of origin discrimination by orc1 initiators in archaea (2010).

Identification and autonomous replication capability of a chromosomal replication origin from the archaeon Sulfolobus solfataricus

A CDC6-like Factor from the Archaea Sulfolobus solfataricus Promotes Binding of the Mini-chromosome Maintenance Complex to DNA

Biochemical evidence of a physical interaction between Sulfolobus solfataricus B-family and Y-family DNA polymerases

Modular organization of a Cdc6-like protein from the crenarchaeon Sulfolobus solfataricus

A novel thermostable protein-tag: optimization of the Sulfolobus solfataricus DNA- alkyl-transferase by protein engineering

The Cdc45·Mcm2–7·GINS Protein Complex in Trypanosomes Regulates DNA Replication and Interacts with Two Orc1-like Proteins in the Origin Recognition Complex

Biochemical characterization of two Cdc6/ORC1-like proteins from the crenarchaeon Sulfolobus solfataricus