NDLI: Promoters from a cold-adapted bacterium: definition of a consensus motif and molecular characterization of UP regulative elements

Content Provider	Springer Nature Link
Author	Duilio, Angela Madonna, Stefania Tuti, Maria Luisa Pirozzi, Marinella Sannia, Giovanni Mari, Gennaro
Copyright Year	2004
Abstract	Although low-temperature tolerant micro-organisms were discovered long ago, limited information is still available on the transcription machinery in cold-adapted bacteria. This knowledge represents a necessary background for the investigation of the adaptation of gene-expression mechanisms at low temperatures. The recent development of a shuttle genetic system for the transformation of the cold-adapted Gram-negative bacterium Pseudoalteromonas haloplanktis strain TAC125 has made possible the isolation of the psychrophilic promoters described in this paper. TAC125 genomic DNA fragments were cloned in the shuttle vector and the promoter-containing recombinant clones were selected for their ability to express a promoter-less lacZ gene. The nucleotide sequence of several selected inserts and the transcription start points of the transcribed m-RNAs were determined. A promoter consensus sequence for Pseudoalteromonas haloplanktis TAC125 was proposed on the basis of a sequence comparison between the various active promoters. Furthermore, the identification and the functional characterization of two UP elements from this cold-adapted bacterium are also reported.
Starting Page	125
Ending Page	132
Page Count	8
File Format	PDF
ISSN	14310651
Journal	Extremophiles
Volume Number	8
Issue Number	2
e-ISSN	14334909
Language	English
Publisher	Springer-Verlag
Publisher Date	2004-01-21
Publisher Place	Tokyo
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Adaptation to cold Promoter consensus sequence Pseudoalteromonas haloplanktis Psychrophilic bacteria UP regulative sequence
Content Type	Text
Resource Type	Article
Subject	Medicine Molecular Medicine Microbiology

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