NDLI: Nuclear DNA content of Pongamia pinnata L. and genome size stability of in vitro-regenerated plantlets

Content Provider	Springer Nature Link
Author	Choudhury, Rimjhim Roy Basak, Supriyo Ramesh, Aadi Moolam Rangan, Latha
Copyright Year	2013
Abstract	Pongamia pinnata L. is a multipurpose versatile legume that is well known as a prospective feedstock biodiesel species. However, to date, there has been little genomic research aimed at the exploitation of the biotechnological potential of this species. Genetic characterization of any plant is a challenging task when there is no information about the genome size and organization of the species. Therefore, the genome size of P. pinnata was estimated by flow cytometry with respect to two standards (Zea mays and Pisum sativum), and compared with that of in vitro-raised plants (nodal segment, in vitro-rooted plantlets and acclimatized in vitro plants) to study the potential effect of somaclonal variation on genome size. This method can be used to support the establishment of true-to-type plants to encourage afforestation programs. Modified propidium iodide/hypotonic citrate buffer was used for isolation of the intact nuclei. The 2C DNA value of this species was estimated to be 2.51 ± 0.01 pg. Statistically, there was no significant difference in the DNA content of the in vitro-grown plants and mother plant at α = 0.05. As a result of the low genome size of P. pinnata, a species that has adapted itself to a wide range of edaphic and ecological condition, we can now proceed for its next generation sequencing and genomic diversity studies.
Starting Page	703
Ending Page	709
Page Count	7
File Format	PDF
ISSN	0033183X
Journal	Protoplasma
Volume Number	251
Issue Number	3
e-ISSN	16156102
Language	English
Publisher	Springer Vienna
Publisher Date	2013-08-29
Publisher Place	Vienna
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Biodiesel Flow cytometry Genome size In vitro culture Pongamia pinnata Cell Biology Plant Sciences Zoology
Content Type	Text
Resource Type	Article
Subject	Cell Biology Plant Science Medicine

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