NDLI: Nuclear DNA content of Hydrastis canadensis L. and genome size stability of in vitro regenerated plantlets

Content Provider	Springer Nature Link
Author	Obae, Samuel G. West, Todd P.
Copyright Year	2010
Abstract	Knowing the genome size is an important step towards deciding and planning for genome sequencing of a given species. Using flow cytometry, nuclear DNA content of Hydrastis canadensis was estimated, and genome size stability of its in vitro regenerated plantlets were assessed. The nuclear DNA content of H. canadensis was estimated to be 2.62 ± 0.020 pg/2C. This is the first report to estimate nuclear DNA content of H. canadensis; therefore this study provides valuable information that will facilitate genome sequencing and subsequent molecular studies of this economically important medicinal plant. Comparison of genome size between in vitro regenerated (explant source was from wild plants) and wild plants of H. canadensis did not reveal any significant difference (P ≤ 0.05) in nuclear DNA content. This suggests that micropropagation of H. canadensis, even after numerous sub-culturing and long-term culture periods produced in vitro plantlets with a stable genome size. These results provide further evidence that micropropagation techniques have the potential to be used as a source of planting stock, along with seeds, for restoring locally threatened H. canadensis wild populations and for commercial cultivation to supply the growing herbal market.
Starting Page	259
Ending Page	263
Page Count	5
File Format	PDF
ISSN	01676857
Journal	Plant Cell, Tissue and Organ Culture (PCTOC)
Volume Number	102
Issue Number	2
e-ISSN	15735044
Language	English
Publisher	Springer Netherlands
Publisher Date	2010-03-04
Publisher Place	Dordrecht
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Germplasm conservation Medicinal plant Goldenseal Yellow root Flow cytometry Tissue culture Plant Pathology Plant Genetics & Genomics Plant Physiology Plant Sciences
Content Type	Text
Resource Type	Article
Subject	Horticulture

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