NDLI: An efficient in planta transformation of Jatropha curcas (L.) and multiplication of transformed plants through in vivo grafting

Content Provider	Springer Nature Link
Author	Jaganath, Balusamy Subramanyam, Kondeti Mayavan, Subramanian Karthik, Sivabalan Elayaraja, Dhandapani Udayakumar, Rajangam Manickavasagam, Markandan Ganapathi, Andy
Copyright Year	2013
Abstract	An efficient and reproducible Agrobacterium-mediated in planta transformation was developed in Jatropha curcas. The various factors affecting J. curcas in planta transformation were optimized, including decapitation, Agrobacterium strain, pin-pricking, vacuum infiltration duration and vacuum pressure. Simple vegetative in vivo cleft grafting method was adopted in the multiplication of transformants without the aid of tissue culture. Among the various parameters evaluated, decapitated plants on pin-pricking and vacuum infiltrated at 250 mmHg for 3 min with the Agrobacterium strain EHA 105 harbouring the binary vector pGA 492 was proved to be efficient in all terms with a transformation efficiency of 62.66 %. Transgene integration was evinced by the GUS histochemical analysis, and the GUS positive plants were subjected to grafting. Putatively transformed J. curcas served as "Scion" and the wild type J. curcas plant severed as "Stock". There was no occurrence of graft rejection and the plants were then confirmed by GUS histochemical analysis, polymerase chain reaction (PCR) and Southern hybridization. Genetic stability of the grafted plants was evaluated by using randomly amplified polymorphic DNA (RAPD), marker which showed 100 % genetic stability between mother and grafted plants. Thus, an efficient in planta transformation and grafting based multiplication of J. curcas was established.
Starting Page	591
Ending Page	601
Page Count	11
File Format	PDF
ISSN	0033183X
Journal	Protoplasma
Volume Number	251
Issue Number	3
e-ISSN	16156102
Language	English
Publisher	Springer Vienna
Publisher Date	2013-10-23
Publisher Place	Vienna
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Agrobacterium tumefaciens EHA105 Cleft grafting Genetic fidelity In planta transformation PPT Cell Biology Plant Sciences Zoology
Content Type	Text
Resource Type	Article
Subject	Cell Biology Plant Science Medicine

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