NDLI: Expression of mouse beta-defensin-3 in MDCK cells and its anti-influenza-virus activity

Content Provider	Springer Nature Link
Author	Jiang, Yan Wang, Yueling Kuang, Yu Wang, Baoning Li, Wanyi Gong, Tianxiang Jiang, Zhonghua Yang, De Li, Mingyuan
Copyright Year	2009
Abstract	Influenza (flu) pandemics have presented a threat to human health in the past century. Because of outbreaks of avian flu in humans in some developing countries in recent years, humans are more eager to find a way to control flu. Mammalian beta-defensins (β-defensins) are associated primarily with mucosal and skin innate immunity. Previous studies have demonstrated antimicrobial properties of a variety of defensin peptides. We have identified the presence of mouse β-defensin 1, 2, and 3 genes (Mbd-1, 2, and 3) in trachea and lung tissues by RT-PCR before and after infection with influenza virus. We constructed a eukaryotic expression plasmid containing Mbd-3, pcDNA 3.1(+)/MBD-3, and the plasmid was introduced into Madin–Darby canine kidney (MDCK) cells by transfection. The expression of Mbd-3 in MDCK cells was verified by immunofluorescence test, RT-PCR, and Western blot. The pcDNA 3.1(+)/MBD-3 plasmid was injected into mice to observe its effect against influenza A virus (IAV) in vivo. Mouse β-defensin genes could be expressed in trachea and lung tissues before IAV infection, but expression of Mbd-2 and Mbd-3 was increased significantly after IAV infection. The survival rate of mice with MBD-3 against IAV challenge was 71.43%, and MDCK cells with MBD-3 could clearly inhibit IAV replication. The results demonstrated that mouse β-defensins possess anti-influenza virus activity, suggesting that mouse β-defensins might be used as agents to prevent and treat influenza.
Starting Page	639
Ending Page	647
Page Count	9
File Format	PDF
ISSN	03048608
Journal	Archives of Virology
Volume Number	154
Issue Number	4
e-ISSN	14328798
Language	English
Publisher	Springer Vienna
Publisher Date	2009-03-20
Publisher Place	Vienna
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Infectious Diseases Medical Microbiology Virology
Content Type	Text
Resource Type	Article
Subject	Virology Medicine

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Original article expression of mouse beta-defensin-3 in mdck cells and its anti-influenza-virus activity.

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