NDLI: Recombinant mouse beta-defensin 2 inhibits infection by influenza A virus by blocking its entry

Content Provider	Springer Nature Link
Author	Gong, Tianxiang Jiang, Yan Wang, Yueling Yang, De Li, Wanyi Zhang, Qiang Feng, Wei Wang, Baoning Jiang, Zhonghua Li, Mingyuan
Copyright Year	2010
Abstract	Human influenza A virus (IAV) is a major cause of life-threatening respiratory tract disease worldwide. Defensins are small cationic peptides of about 2–6 kDa that are known for their broad-spectrum antimicrobial activity. Here, we focused on the anti-influenza A activity of mouse β-defensin 2 (mBD2). The prokaryotic expression plasmid pET32a-mBD2 was constructed and introduced into Escherichia coli Rosseta gami (2) to produce recombinant mBD2 (rmBD2). Purified rmBD2 showed strong antiviral activity against IAV in vitro. The protective rate for Madin–Darby canine kidney cells was 93.86% at an rmBD2 concentration of 100 μg/ml. Further studies demonstrated that rmBD2 prevents IAV infection by inhibiting viral entry. In addition, both pretreatment and postinfection treatment with rmBD2 provided protection against lethal virus challenge with IAV in experimental mice, with protection rates of 70 and 30%, respectively. These results suggest that the mBD2 might have important effects on influenza A virus invasion.
Starting Page	491
Ending Page	498
Page Count	8
File Format	PDF
ISSN	03048608
Journal	Archives of Virology
Volume Number	155
Issue Number	4
e-ISSN	14328798
Language	English
Publisher	Springer Vienna
Publisher Date	2010-03-02
Publisher Place	Vienna
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Infectious Diseases Medical Microbiology Virology
Content Type	Text
Resource Type	Article
Subject	Virology Medicine

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1	Ministry of Education (GoI), Department of Higher Education	Sanctioning Authority	https://www.education.gov.in/ict-initiatives
2	Indian Institute of Technology Kharagpur	Host Institute of the Project: The host institute of the project is responsible for providing infrastructure support and hosting the project	https://www.iitkgp.ac.in
3	National Digital Library of India Office, Indian Institute of Technology Kharagpur	The administrative and infrastructural headquarters of the project	Dr. B. Sutradhar bsutra@ndl.gov.in
4	Project PI / Joint PI	Principal Investigator and Joint Principal Investigators of the project	Dr. B. Sutradhar bsutra@ndl.gov.in Prof. Saswat Chakrabarti will be added soon
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