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| Content Provider | Springer Nature Link |
|---|---|
| Author | Roy, A. Ramachandran, P. Brlansky, R. H. |
| Copyright Year | 2002 |
| Abstract | Citrus tristeza virus (CTV) is an aphid-transmitted closterovirus, which causes one of the most important citrus diseases worldwide. Isolates of CTV differ widely in their biological properties. CTV-infected samples were collected from four locations in India: Bangalore (CTV-B), Delhi (CTV-D), Nagpur (CTV-N), and Pune (CTV-P), and were maintained by grafting into Kagzi lime (Citrus aurantifolia (Christm. Swing.). All isolates produced typical vein clearing and flecking symptoms 6–8 weeks after grafting. In addition, CTV-B and CTV-P isolates produced stem-pitting symptoms after 8–10 months. The CTV coat protein gene (CPG) was amplified by RT-PCR using CPG specific primers, yielding an amplicon of 672 bp for all the isolates. Sequence analysis of the CPG amplicon of all the four Indian isolates showed 93–94% nucleotide sequence homology to the Californian CTV severe stem pitting isolate SY568 and 92–93% homology to the Japanese seedling yellows isolate NUagA and Israeli VT p346 isolates. In phylogenetic tree analysis, Indian CTV isolates appeared far different from other isolates as they formed a separate branch. Comparison among the Indian isolates was carried out by restriction analysis and restriction fragment length polymorphism (RFLP). Specific primers to various genome segments of well-characterized CTV isolates were used to further classify the Indian CTV isolates. |
| Starting Page | 707 |
| Ending Page | 722 |
| Page Count | 16 |
| File Format | |
| ISSN | 03048608 |
| Journal | Archives of Virology |
| Volume Number | 148 |
| Issue Number | 4 |
| e-ISSN | 14328798 |
| Language | English |
| Publisher | Springer-Verlag |
| Publisher Date | 2003-03-01 |
| Publisher Place | Wien |
| Access Restriction | One Nation One Subscription (ONOS) |
| Content Type | Text |
| Resource Type | Article |
| Subject | Virology Medicine |
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