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Characterization of Citrus tristeza virus strains from southern China based on analysis of restriction patterns and sequences of their coat protein genes
| Content Provider | Semantic Scholar |
|---|---|
| Author | Jiang, Bo Wang, Guo-Ping Hu, John Zhang, Jian-Kun Wang, Caixia Liu, Yong Fan, Xu-Dong |
| Copyright Year | 2008 |
| Abstract | Citrus tristeza virus (CTV) isolates collected from southern China were characterized by biological indexing on citrus indicators, restriction fragment length polymorphism (RFLP), and bi-directional reverse transcription-polymerase chain reaction (BD-PCR) analysis of their coat protein (CP) genes. Of the 30 isolates, only two isolates, N3 and N4, did not induce visible symptoms. Twenty-eight other isolates induced stem pitting and vein clearing, plant stunting, and leaf yellowing symptoms on Mexican lime, Duncan grapefruit, and sour orange seedlings. In BD-PCR analysis, a 392-bp fragment specific for the mild strains was amplified from isolates N3 and N4, and a 320-bp fragment specific for the severe strains was produced from the other 28 isolates. The RFLP analysis for RT-PCR products of the CP gene with restriction enzyme HinfI identified seven groups representing groups I–VI and a new group, which was not involved in the seven groups defined by Gillings (J Virol Methods 44:305–317, 1993). The sequences of the CP genes from 12 Chinese CTV isolates showed a high divergence, with 91.5–99.7% identities at the nucleotide level and 94.2–99.6% identities at the amino acid level. Our results suggest that the composition of CTV populations from China has a high genetic diversity in the CP gene. |
| Starting Page | 185 |
| Ending Page | 192 |
| Page Count | 8 |
| File Format | PDF HTM / HTML |
| DOI | 10.1007/s11262-008-0254-9 |
| Alternate Webpage(s) | http://www2.hawaii.edu/~johnhu/200802.pdf |
| PubMed reference number | 18626763 |
| Alternate Webpage(s) | https://doi.org/10.1007/s11262-008-0254-9 |
| Journal | Medline |
| Volume Number | 37 |
| Journal | Virus Genes |
| Language | English |
| Access Restriction | Open |
| Content Type | Text |
| Resource Type | Article |
