NDLI: Label-free supersandwich electrogenerated chemiluminescence biosensor for the determination of the HIV gene

Content Provider	Springer Nature Link
Author	Ruan, Sanpeng Li, Zhejian Qi, Honglan Gao, Qiang Zhang, Chengxiao
Copyright Year	2014
Abstract	We describe a highly sensitive electrochemiluminescence (ECL) based method for the determination of the human immunodeficiency virus-1 (HIV-1) gene. A long-range self-assembled double strand DNA (ds-DNA) is used as a carrier, and the ruthenium complex Ru(phen)3 2+ as an ECL indicator for signal amplification. The thiolated ss-DNA serving as a capture probe is firstly self-assembled on the surface of a gold electrode. After the target HIV-1 gene is completely hybridized with the capture probe, two previously hybridized auxiliary probes are hybridized with the target HIV-1 gene to form long-range supersandwich ds-DNA polymers on the surface of the electrode. Finally, the ECL indicator is intercalated into the supersandwich ds-DNA grooves. This results in a strongly increased ECL in tripropylamine solution because a large fraction of the intercalator is intercalated into supersandwich ds-DNA. The results showed that the increased ECL intensity is directly related to the logarithm of the concentration of the HIV-1 gene in the range from 0.1 pM to 0.1 nM, with a detection limit of 0.022 pM and using only 10 μL of analyte samples. The method can effectively discriminate target HIV-1 gene (a perfectly matched ss-DNA) from a 2-base mismatched ss-DNA. This work demonstrates that the high sensitivity and selectivity of an ECL DNA biosensor can be largely improved by using supersandwich ds-DNA along with ECL indicators. Figure The method can effectively discriminate target HIV-1 gene (a perfectly matched ss-DNA) from a 2-base mismatched ss-DNA. The high sensitivity and selectivity of electrochemiluminescence DNA biosensor can be largely improved by using supersandwich ds-DNA along with ECL indicators.
Starting Page	1293
Ending Page	1300
Page Count	8
File Format	PDF
ISSN	00263672
Journal	Microchimica Acta
Volume Number	181
Issue Number	11-12
e-ISSN	14365073
Language	English
Publisher	Springer Vienna
Publisher Date	2014-04-16
Publisher Place	Vienna
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	DNA hybridization Electrogenerated chemiluminescence Human immunodeficiency virus-1 gene Supersandwich model Nanochemistry Nanotechnology Characterization and Evaluation of Materials Analytical Chemistry Microengineering
Content Type	Text
Resource Type	Article
Subject	Analytical Chemistry

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